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Genetic diversity of the coat protein of olive latent virus 1 isolates
Varanda, Carla; Nolasco, Gustavo; Clara, Maria Ivone; Félix, Maria RosárioThe CP gene variability among 21 olive latent virus 1 (OLV-1) isolates obtained from different hosts and locations and at different times was assessed. Amplicons obtained by RT-PCR were cloned, and at least 10 sequences from each isolate were analyzed and compared. OLV-1 sequences available in GenBank were included. The encoded CPs consisted of 270 amino acids, except those of isolates G1S and C7 (269 aa) and G...
A reassessment of the anamorphic fungus Fusicoccum luteum and description of it...
Phillips, A. J. L.; Fonseca, Filomena; Povoa, V.; Castilho, Rita; Nolasco, GustavoWhen Fusicoccum luteum was first described in 1985, it was not found sporulating in nature and its teleomorph was not known. Recently, a Botryosphaeria sp. was found on grapevines in Portugal, and the anamorph in culture resembled F. luteum. Nucleotide sequence of the nuclear rDNA internal transcribed spacer ITS2 distinguished isolates of this fungus from others in the B. dothidea complex, and the teleomorph, B...
Técnicas biomoleculares no diagnòstico e tipificação em virologia vegetal
Nolasco, GustavoTese de dout., Ciências Agrárias (Protecção de Plantas), Unidade de Ciências e Tecnologias Agrárias, Univ. do Algarve, 1994 ; O diagnóstico é um dos principais meios de protecção de plantas em virologia vegetal. Muito frequentemente seria vantajoso poder ainda complementar o diagnóstico com informação sobre as estirpes que estão presentes num dado contexto epidemiológico. Na maior parte dos casos isto não é po...
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