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New improved method for fructooligosaccharides production by Aureobasidium pull...

Dominguez, Ana; Nobre, C.; Rodrigues, L. R.; Peres, A. M.; Torres, D.; Rocha, I.; Lima, Nelson; Teixeira, J. A.

Fructooligosaccharides are prebiotics with numerous health benefits within which the improvement of gut microbiota balance can be highlighted, playing a key role in individual health. In this study, an integrated one-stage method for FOS production via sucrose fermentation by Aureobasidium pullulans was developed and optimized using experimental design tools. Optimization of temperature and agitation speed for ...


Fructo-oligosaccharides purification from a fermentative broth using an activat...

Nobre, C.; Teixeira, J. A.; Rodrigues, L. R.

In this study, a simple and efficient process to purify fructo-oligosaccharides (FOS) from a fermentative broth was proposed using a single activated charcoal column. The FOS adsorption onto the activated charcoal was modeled by a pseudo-second order model. Several volumes and concentrations of water/ ethanol were studied to optimize the selective desorption of sugars from the broth mixture at 25 8C. Mixtures c...


Operating conditions of a simulated moving bed chromatography unit for the puri...

Nobre, C.; Severino, Antoni; Retamal, Cristina; De Weireld, Guy; Wouwer, Alain Van de; Teixeira, J. A.; Rodrigues, L. R.


Purification of fructo-oligosaccharides

Nobre, C.; Teixeira, J. A.; Rodrigues, L. R.

Este resumo faz parte de: Book of abstracts of the Meeting of the Institute for Biotechnology and Bioengineering, 2, Braga, Portugal, 2010. A versão completa do livro de atas está disponível em: http://hdl.handle.net/1822/10968


Comparison of adsorption equilibrium of fructose, glucose and sucrose on potass...

Nobre, C.; Santos, Maria João; Dominguez, Ana; Torres, D.; Rocha, Orlando; Peres, A. M.; Rocha, I.; Ferreira, E. C.; Teixeira, J. A.; Rodrigues, L. R.

Adsorption equilibrium of fructose, glucose and sucrose was evaluated on sulfonated poly(styrene-co-divinylbenzene) cation-exchange resins. Two types of resins were used: potassium (K+) gel-type and sodium (Na+) macroporous resins. Influence of the cation and effect of the resin structure on adsorption were studied. The adsorption isotherms were determined by the static method in batch mode for mono-component a...


A dynamical model for the fermentative production of fructooligosaccharides

Rocha, Orlando; Nobre, C.; Dominguez, Ana; Torres, D.; Faria, Nuno; Rodrigues, L. R.; Teixeira, J. A.; Ferreira, E. C.; Rocha, I.

In this paper a detailed mathematical model is presented for the fermentative production of fructo-oligosaccharides with Aspergillus sp. The model accounts for hydrolysis and transfructolization reactions, as well as biomass formation and it contains 27 parameters that were determined from experimental data using a System Biology toolbox with the Simulated Annealing method for curve fitting. Several additional ...


Adsorption equilibrium of fructose, glucose and sucrose for cationic resins in ...

Nobre, C.; Santos, Maria João; Dominguez, Ana; Torres, D.; Peres, A. M.; Rocha, I.; Ferreira, E. C.; Rodrigues, L. R.; Teixeira, J. A.


Determination of frutooligosaccharides adsorption parameters using ion-exchange...

Nobre, C.; Dominguez, Ana; Torres, D.; Rocha, O.; Rocha, I.; Teixeira, J. A.; Ferreira, E. C.; Rodrigues, L. R.

Fructooligosaccharides (FOS) are non-digestible sugars that beneficially affect the host by stimulating the growth of specific bacteria in the colon. In large scale, FOS can be produced from sucrose by fermentation. The fermentative broth obtained from this process is a complex mixture of salts and sugars. These sugar mixtures include FOS, namely kestose (GF2), nystose (GF3) and fructo-furanosylnystose (GF4), b...


Optimization of a fructooligosaccharides purification method using activated ch...

Nobre, C.; Dominguez, Ana; Torres, D.; Rocha, Orlando; Rocha, I.; Ferreira, E. C.; Teixeira, J. A.; Rodrigues, L. R.

Fructooligosaccharides (FOS) have gained large commercial interest due to its beneficial properties in the human health as prebiotics. FOS are produced industrially by fermentative processes. However, the result of such fermentations is a complex mixture containing salts and approximately 50% (w/w) of low molecular weight sugars that have to be eliminated. Among other techniques that have been studied, the adso...


Fractionation of fructo-oligosaccharides by gel filtration

Nobre, C.; Santos, Maria João; Dominguez, Ana; Torres, D.; Rodrigues, L. R.; Rocha, I.; Teixeira, J. A.; Ferreira, E. C.


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Fundação para a Ciência e a Tecnologia Universidade do Minho   Governo Português Ministério da Educação e Ciência Programa Operacional da Sociedade do Conhecimento União Europeia