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Phenotypic differentiation and genetic diversity of Saccharomyces cerevisiae st...
Neves, J. Drumonde; Lima, Maria Teresa; Schuller, DoritThis work aims to evaluate diversity of Saccharomyces cerevisiae strains from vineyards of the Azores Archipelago and to estimate the degree of phenotypic and genetic differentiation among geographically isolated islands. During two consecutive years, 163 grape musts were obtained from grape samples collected in eight islands of the Archipelago, that belonged to traditional (Arinto, Verdelho and Terrantez) and ...
Yeast biodiversity in the vineyards of the Azores Archipelago (Portugal)
Neves, J. Drumonde; Lima, Teresa; Schuller, DoritThis study aims to identify the fermentative yeasts communities of the vineyards from the mid-Atlantic islands ecosystems of the Azores Archipelago and to evaluate whether the populations vary according to the islands geography, grape variety and type of vineyard. From an ecological point of view, vineyards of these isolated volcanic islands terroirs correspond to very particular ecosystems, whose yeast flora i...
Study of genetic and phenotypic relationships in a Saccharomyces cerevisiae str...
Duarte, Ricardo Franco; Mendes, Inês; Umek, Lan; Neves, J. Drumonde; Zupan, Blaz; Schuller, DoritGenome sequencing is essential to understand individual variation and to study the relationship between genotype and phenotype. Recently, large-scale sequencing projects of Saccharomyces cerevisiae revealed the existence of a few well defined lineages and some mosaics of that lineages, and suggested the occurrence of two domestication events during the history of association to human activities, one for sake st...
The phenotypic landscape of a Saccharomyces cerevisiae strain collection
Mendes, Inês; Duarte, Ricardo Franco; Umek, Lan; Fonseca, Elza; Neves, J. Drumonde; Dequin, Sylvie; Zupan, Blaz; Schuller, DoritWithin our previous work [1] we developed computational models to predict strains with specific phenotypes (e.g. low ethanol resistance, growth at 30ºC and growth in media containing galactose, raffinose or urea) from microsatellite allelic patterns. The objective of the present work was to gain deeper understanding of the phenotypic diversity of a heterogeneous Saccharomyces cerevisiae strain collection, using...
Genotypic and pheno-metabolomic characterization of a Saccharomyces cerevisiae ...
Duarte, Ricardo Franco; Mendes, Inês; Castro, C. C.; Silva, J. S.; Xavier, A. L.; Neves, J. Drumonde; Oliveira, C.; Martins, Rui C.; Oliveira, J. M.The objective of the present work was to gain a deeper understanding of the phenotypic diversity of natural isolates of Saccharomyces cerevisiae, using high-throughput methods in combination with bioanalytical data. A S. cerevisiae strain collection was constituted comprising 187 strains from different geographical origins and technological uses. Some microsatellite alleles and phenotypes were identified as res...
The biogeography of fermentative yeast populations from the vineyards of the Az...
Neves, J. Drumonde; Lima, Maria Teresa; Schuller, DoritDuring the harvests of 2009 and 2010, 163 musts were obtained from grape samples collected in eight islands of the Archipelago of the Azores. The sampling plan included traditional and hybrid grape varieties from vineyards in appellations of origin and abandoned vineyards. In 2009, 2640 yeast isolates were obtained from the initial stage of fermentation, belonging to more than 20 species. Differences between mo...
The biogeography of fermentative yeast populations from the vineyards of the Az...
Neves, J. Drumonde; Lima, Maria Teresa; Schuller, DoritDuring the harvests of 2009 and 2010, 163 musts were obtained from grape samples collected in eight islands of the Archipelago of the Azores. The sampling plan included traditional and hybrid grape varieties from vineyards in appellations of origin and abandoned vineyards. In 2009, 2640 yeast isolates were obtained from the initial stage of fermentation, belonging to more than 20 species. Differences between mo...
A biogeografia de populações de leveduras fermentativas das vinhas do arquipéla...
Neves, J. Drumonde; Lima, Maria Teresa; Schuller, DoritNas vindimas de 2009 e 2010 foram obtidos 163 mostos de amostras de uvas colhidas em oitos ilhas do Arquipélago dos Açores. A amostragem incluiu castas tradicionais, e híbridas a partir de vinhas em zonas demarcadas e não demarcadas e vinhas abandonadas. Em 2009 obtiveram-se 2640 isolados de levedura da fase inicial da fermentação, que pertenceram a mais de 20 espécies. Relativamente às espécies mais representa...
The biogeography of fermentative yeast populations from the vineyards of the Az...
Neves, J. Drumonde; Lima, Maria Teresa; Schuller, DoritThis study aims at the characterization of the fermentative yeasts flora of the vineyards from the Azores archipelago, for biodiversity preservation and the constitution of a Saccharomyces cerevisiae strain collection. During the harvests of 2009 and 2010, 163 (88 and 75) grape samples were collected from eight islands of the archipelago, that belonged to traditional (Arinto, Verdelho and Terrantez) and hybrid ...
A new high-throughput method for the rapid extraction of yeast DNA
Vieira, E.; Neves, J. Drumonde; Lima, Maria Teresa; Araújo, Isabel M.; Casal, Margarida; Schuller, DoritEcological, evolutionary and population genetic studies of yeasts require the processing of a high numbers of isolates. The current molecular methods for yeast identification and characterization require time-consuming and labor intensive DNA extraction protocols. Within this research, we aimed to develop a high-throughput method for yeast DNA extraction. Total yeast DNA extraction was based on a previously des...
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