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Signal transduction profile of chemical sensitisers in dendritic cells: an endp...
Neves, BM; Gonçalo, Margarida; Figueiredo, A; Duarte, CB; Lopes, MC; Cruz, MTThe development of non-animal testing methods for the assessment of skin sensitisation potential is an urgent challenge within the framework of existing and forthcoming legislation. Efforts have been made to replace current animal tests, but so far no alternative methods have been developed. It is widely recognised that alternatives to animal testing cannot be accomplished with a single approach, but rather wil...
Effect of lipopolysaccharide, skin sensitizers and irritants on thioredoxin-1 e...
Francisco, V; Neves, BM; Cruz, MT; Gonçalo, Margarida; Figueiredo, A; Duarte, CB; Lopes, MCThioredoxin-1 is a ubiquitous protein involved in phenotypical and functional changes in dendritic cells (DC). We investigated the effect of lipopolysaccharide (LPS), skin sensitizers, and irritants on thioredoxin-1 by Western blot and immunofluorescence and on mRNA by real-time PCR. As DC models, we used a skin DC line and DC derived from human blood monocytes. We observed that all tested chemicals increased t...
Differential modulation of CXCR4 and CD40 protein levels by skin sensitizers an...
Neves, BM; Cruz, MT; Francisco, V; Gonçalo, Margarida; Figueiredo, A; Duarte, CB; Lopes, MCThe development of non-animal methods for skin sensitization testing is an urgent challenge. Some of the most promising in vitro approaches are based on the analysis of phenotypical and functional modifications induced by sensitizers in dendritic cell models. In this work, we evaluated, for the first time, a fetal skin-derived dendritic cell line (FSDC) as a model to discriminate between sensitizers and irritan...
Effect of skin sensitizers on inducible nitric oxide synthase expression and ni...
Cruz, MT; Neves, BM; Gonçalo, Margarida; Figueiredo, A; Duarte, CB; Lopes, MCNitric oxide (NO) is involved in the pathogenesis of acute and chronic inflammatory conditions, namely in allergic contact dermatitis (ACD). However, the mechanism by which NO acts in ACD remains elusive. The present study focuses on the effects of different contact sensitizers (2,4-dinitrofluorbenzene, 1,4-phenylenediamine, nickel sulfate), the inactive analogue of DNFB, 2,4-dichloronitrobenzene, and two irrit...
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