A PCR-RFLP assay was developed for the identification of trichodorids belonging to Nanidorus, Paratrichodorus and Trichodorus genera. Exploring the variability of the 18S SSU rDNA gene this method provides for the first time a molecular diagnosis tool alternative to the difficult and time consuming phenotyping especially of quite similar species, enabling selectivity in mixed samples with non-trichodorid specie...
A survey of virus vector trichodorid nematodes was carried out in the central and northern regions of Portugal. Morphobio- metric identification showed the presence of trichodorid species previously reported from Portugal, except for Paratrichodorus porosus, which is reported for the first time in Continental Europe. Small subunit ribosomal DNA (SSU rDNA) sequences of ten different species occurring in Portugal...
Resumo do poster apresentado no XXVII ESN International Symposium, Rome, 2004. ; Trichodorid nematodes of the genera Trichodorus and Paratrichodorus are known natural vectors of Tobacco Rattle Virus (TRV) to many agronomically important crops. Previous taxonomic studies have reported several trichodorids, including virus-vector species, from Portugal. The trichodorid group is well defined taxonomically but l...
Some trichodorid species are known to vector specific strains of Tobacco Rattle Virus (TRV) that have a deleterious effect upon several economically important crops, e.g. potato and tobacco. Given the complex relationship that exists between trichodorids and the various TRV strains, it is imperative that a rapid and accurate identification to species level is achieved for the effective use of control measures w...
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