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Effect of pH and temperature on phytase and biomass production by submerged fer...

Nascimento, J. C. S.; Souza, F. A. S. D.; Porto, Tatiana Souza; Mussatto, Solange I.; Teixeira, J. A.; Porto, Ana L. F.

Phytase production and biomass was evaluated in present work by submerged fermentation with Aspergillus niger var. phoenicis URM 4924. Experimental assays were done under different conditions of pH (4.0 to 8.0) and temperature (25 to 35 ºC), and the influence of these variables on the responses was studied through a 22 central composite design and response surface methodology. Phytase and biomass production wer...


Recovery and partitioning of fibrinolytic protease from Bacillus sp. UFPEDA 485...

Souza, F. A. S. D.; Nascimento, J. C. S.; Marques, D. A. V.; Marcos, João Carlos; Porto, T. S.; Teixeira, J. A.; Porto, A .L .F.

Fibrinolytic proteases produced by Bacillus sp. has attracted interest in the pharmaceutical industry as a promising alternative in thrombolytic therapy due to their effectiveness in degrading fibrin, its production requiring the development of an efficient recovery process. Aqueous two-phase systems (ATPS) have been recognized as an efficient and economical process for recovering enzymes. To optimize the recov...


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Fundação para a Ciência e a Tecnologia Universidade do Minho   Governo Português Ministério da Educação e Ciência Programa Operacional da Sociedade do Conhecimento União Europeia