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A substrate translocation trajectory in a cytoplasm-facing topological model of...
Silva, Isabel Soares; Pessoa, Joana Sá; Myrianthopoulos, Vassilios; Mikros, Emmanuel; Casal, Margarida; Diallinas, GeorgePrevious mutational analysis of Jen1p, a Saccharomyces cerevisiae monocarboxylate/H+ symporter of the Major Facilitator Superfamily, has suggested that the consensus sequence 379NXX[S/T]HX[S/T]QD387 in transmembrane segment VII (TMS-VII) is part of the substrate translocation pathway. Here, we rationally design, analyse and show that several novel mutations in TMS-V and TMS-XI directly modify Jen1p function. Am...
A substrate translocation trajectory in the monocarboxylate/h+ symporter jen1
Pessoa, Joana Sá; Silva, Isabel Soares; Myrianthopoulos, Vassilios; Mikros, Emmanuel; Diallinas, George; Casal, MargaridaPrevious mutational analysis of Jen1p, a Saccharomyces cerevisiae monocarboxylate/H+ symporter of the Major Facilitator Superfamily, has suggested that the consensus sequence 379NXX[S/T]HX[S/T]QD387, located in transmembrane segment VII (TMS-VII), is part of the substrate translocation pathway. In this work, we rationally design and analyse novel mutations concerning residues in TMS-V and TMS-XI. Our analysis i...
Identification of amino acid residues critical for the substrate translocation ...
Pessoa, Joana Sá; Silva, Isabel Soares; Myrianthopoulos, Vassilios; Mikros, Emmanuel; Diallinas, George; Casal, MargaridaLactic, acetic and propionic acids have been used for many years in industrial and pharmaceutical companies. In Saccharomyces cerevisiae, Jen1p is a major monocarboxylate:H+ symporter specific primarily for lactate, pyruvate and for acetate (TC # 2.A.1.12.2) (Casal et al., 1999). A phylogenetic tree of ScJen1p homologues (Casal et al., 2008) showed the existence of two main clusters: a Jen1 group (monocarboxyl...
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