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Enzymatic polymerization on the surface of functionalized cellulose fibers

Kim, Suyeon; Zille, Andrea; Murkovic, M.; Guebitz, G. M.; Paulo, Artur Cavaco

Enzymatic coating of functionalized cellulose fibers with catechol was performed in the presence of Trametes hirsuta laccase. Cellulose functionalization was done by covalent fixation of aromatic amines onto the cellulose surface using a dyeing procedure with C.I. Reactive Black 5 (RB5) followed by reduction with sodium hydrosulfite. Cellulase enzymes were used on coated and control samples to obtain the analyt...


Enzymatic reduction of azo and indigoid compounds

Pricelius, S.; Held, C.; Murkovic, M.; Bozic, M.; Kokol, V.; Paulo, Artur Cavaco; Gübitz, Georg M.

A customer- and environment-friendly method for the decolorization azo dyes was developed. Azoreductases could be used both to bleach hair dyed with azo dyes and to reduce dyes in vat dyeing of textiles. A new reduced nicotinamide adenine dinucleotide-dependent azoreductase of Bacillus cereus, which showed high potential for reduction of these dyes, was purified using a combination of ammonium sulfate precipita...


Enzymatic reduction and oxidation of fibre-bound azo-dyes

Pricelius, S.; Held, C.; Sollner, S.; Deller, S.; Murkovic, M.; Ullrich, R.; Hofrichter, M.; Paulo, Artur Cavaco; Macheroux, P.; Guebitz, G. M.

A new customer and environmental friendly method of hair bound dye decolouration was developed. Biotransformation of the azo-dyes Flame Orange and Ruby Red was studied using different oxidoreductases. The pathways of azo dye conversion by these enzymes were investigated and the intermediates and metabolites were identified and characterised using UV–vis spectroscopy, high-performance liquid chromatography (HPLC...


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    Financiadores do RCAAP

Fundação para a Ciência e a Tecnologia Universidade do Minho   Governo Português Ministério da Educação e Ciência Programa Operacional da Sociedade do Conhecimento União Europeia