Encontrados 13 documentos, a visualizar página 1 de 2
Characterization of ligno-cellulosic materials bleached with oxo-diperoxo-molyb...
Sîrghiea, Cecilia; Bodescu, Adina-Maria; Botar, Alexandru; Paulo, Artur Cavaco; Munteanu, Florentina-DanielaA newly effective system was used to bleach ligno-cellulosic textile materials. This system is based on two different newly synthesized natrium oxo-diperoxo molybdates, Na2[MoO (O2)2(C2O4)] and Na2[MoO (O2)2(C6H6O7)]. These two compounds were characterized by means of cyclic voltammetry, and the bleached fabrics were fully characterized by measuring their whiteness index, percent loss in fabric weight and the ...
Preparation and characterization of CrN Oy thin films : the effect of compos...
Arvinte, R.; Borges, Joel; Sousa, R. E.; Munteanu, Florentina-Daniela; Barradas, N. P.; Alves, E.; Vaz, F.; Marques, L.Metallic oxynitrides have attracted the attention of several researchers in the last decade due to their versatile properties. Through the addition of a small amount of oxygen into a transition metal nitride film, the material’s bonding states between ionic and covalent types can be tailored, thus opening a wide range of electrical, optical, mechanical and tribological responses. Among the o...
Protein disulphide isomerase-assisted functionalization of keratin-based matrices
Fernandes, Margarida M.; Gomes, A. C.; Vasconcelos, Andreia; Munteanu, Florentina-Daniela; Tzanov, Tzanko; Gonçalves, M. Sameiro T.; End, N.In living systems, protein disulphide isomerase (PDI, EC 5.3.4.1) regulates the formation of new disulphide bonds in proteins (oxidase activity) and catalyzes the rearrangement of non-native disulphide bonds (isomerase activity), leading proteins towards their native configuration. In this study, PDI was used to attach cysteine-containing compounds (CCCs) onto hair, to enhance compound migration within hair fib...
Biosensors based on laccase for detection of commercially reactive dyes
Munteanu, Florentina-Daniela; Paulo, Artur CavacoThe results obtained using Trametes versicolor laccase modified graphite electrodes for detection of eleven different commercially reactive dyes are presented for the first time herein. The increase in current upon injection of the analyzed substrate was shown to be approximated by Michaelis–Menten type dependence. The calculated kinetic constants were used to evaluate the applicability of the laccase modified ...
Expression system of CotA-laccase for directed evolution and high-throughput sc...
Brissos, V.; Pereira, Luciana; Munteanu, Florentina-Daniela; Paulo, Artur Cavaco; Martins, L. O.Laccases are useful biocatalysts for many diverse biotechnological applications. In this study we have established efficient and reliable expression systems and high-throughput screenings for the recombinant CotA-laccase from Bacillus subtilis. The expression levels of cotA-laccase were compared in five different Escherichia coli host strains growing in 96-well microtiter plates under different culture conditio...
Bioelectrochemical investigations of aryl-alcohol oxidase from Pleurotus eryngii
Munteanu, Florentina-Daniela; Ferreira, Patrícia; Ruiz-Duenas, Francisco J.; Martínez, Angel T.; Paulo, Artur CavacoArticle in Press ; Aryl-alcohol oxidase (AAO) electrochemistry studies, using graphite-modified electrodes, are presented for the first time herein. The increase in current upon injection of the analyzed substrate was shown to be approximated by Michaelis–Menten type dependence. The calculated kinetic constants were used to characterize the native (non-mutated) recombinant AAO expressed in Escherichia coli, as...
MALDI-TOF mass spectrometry in textile industry
Munteanu, Florentina-Daniela; Dinca, Nicolae; Paulo, Artur CavacoIn this paper are presented the possibilities of using matrix assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometry in textile industry. MALDI-TOF mass spectrometry it is a convenient, versatile method for characterization and identification of dyes and pigments, and also for characterization of fibers and contaminants of the fabrics.
Staining of wool using the reaction products of ABTS oxidation by Laccase : syn...
Munteanu, Florentina-Daniela; Basto, Carlos; Gübitz, Georg M.; Paulo, Artur CavacoThe effects of ultrasound on 2,2′-Azinobis(3-ethylbenzothiazoline-6-sulfonate) enzymatic oxidation by laccase (Trametes villosa) has been studied by means of cyclic voltammetry. The reaction was allowed to proceed in the presence of a piece of wool and the coloration depth of the wool fabric was measured by means of K/S. It was observed that cyclic voltammetry is influenced the dyeing process and higher K/S val...
Sono-enzymatic polymerization of catechol
Fernandes, Margarida M.; Basto, Carlos; Zille, Andrea; Munteanu, Florentina-Daniela; Gübitz, Georg M.; Paulo, Artur Cavaco"Abstracts of papers presented at the 232nd American Chemical Society National Meeting" ; The potential of laccase enzymes for polymerizing, crosslinking and functionalizing various compounds was studied extensively and increasing interest has been focused on the application of this enzyme as a new biocatalyst in organic synthesis.[1-6] Laccases (EC 1.10.3.2) are a class of multi-copper-containing oxidoreducta...
Laccase kinetics of degradation and coupling reactions
Zille, Andrea; Munteanu, Florentina-Daniela; Gübitz, Georg M.; Paulo, Artur CavacoThe ability of Trametes villosa laccase to degrade an azo dye (methyl orange) and to catalyze the coupling reactions between the aromatic amines and catechol was studied. It was found that the apparent Michaelis–Menten constants obtained from amperometric measurements were comparable to the ones obtained in the oxygen consumption experiments (8.20 and 7.40 µM, respectively). From the measurements of the mediate...
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