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Drosophila S2 cells as a model system to investigate mitotic spindle dynamics, ...
Moutinho-Pereira, Sara; Matos, Irina; Maiato, HelderIn order to perpetuate their genetic content, eukaryotic cells have developed a microtubule-based machine known as the mitotic spindle. Independently of the system studied, mitotic spindles share at least one common characteristic – the dynamic nature of microtubules. This property allows the constant plasticity needed to assemble a bipolar structure, make proper kinetochoremicrotubule attachments, segregate ch...
Microtubule Cytoskeleton Remodeling by Acentriolar Microtubule-organizing Cente...
Moutinho-Pereira, Sara; Debec, Alain; Maiato, HelderCytoskeleton microtubules undergo a reversible metamorphosis as cells enter and exit mitosis to build a transient mitotic spindle required for chromosome segregation. Centrosomes play a dominant but dispensable role in microtubule (MT) organization throughout the animal cell cycle, supporting the existence of concurrent mechanisms that remain unclear. Here we investigated MT organization at the entry and exit f...
Dual Role of Topoisomerase II in Centromere Resolution and Aurora B Activity
Coelho, Paula A.; Queiroz-Machado, Joana; Carmo, Alexandre M.; Moutinho-Pereira, Sara; Maiato, Helder; Sunkel, Claudio E.Chromosome segregation requires sister chromatid resolution. Condensins are essential for this process since they organize an axial structure where topoisomerase II can work. How sister chromatid separation is coordinated with chromosome condensation and decatenation activity remains unknown. We combined four-dimensional (4D) microscopy, RNA interference (RNAi), and biochemical analyses to show that topoisomera...
The ultrastructure of the kinetochore and kinetochore fiber in Drosophila somat...
Maiato, Helder; Hergert, Polla J.; Moutinho-Pereira, Sara; Dong, Yimin; VandenBeldt, Kristin J.; Rieder, Conly L.; McEwen, Bruce F.Drosophila melanogaster is a widely used model organism for the molecular dissection of mitosis in animals. However, despite the popularity of this system, no studies have been published on the ultrastructure of Drosophila kinetochores and kinetochore fibers (K-fibers) in somatic cells. To amend this situation, we used correlative light (LM) and electron microscopy (EM) to study kinetochores in cultured Drosoph...
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