Medicina ; Medicine
Analysis of the expression of S alleles in ‘Rocha’ pear. In this work, the expression of incompatibility genes (S-alleles) in ‘Rocha’ pear was investigated. The comparison of these alleles with the alleles of other cultivars may constitute a tool to help the technicians and farmers to choose pollinators when planning a new ‘Rocha’ orchard. We have used molecular techniques that include pistil RNA extraction fro...
In order to select pear cultivar which are genetically compatible with ‘Rocha’ (Sa, Sj), the identification of S alleles sequences of commercial cultivars with bloom periods overlapping (partially or totally) the bloom period of ‘Rocha’ was performed. Partial sequences of the S alleles from the different cultivars were amplified with specific primers, cloned and sequenced. ‘Pêra De Água’ (S22, Sb) ‘Général Lecl...
To clarify the role of ammonium transporters in N nutrition of ‘Rocha’ pear trees grafted on ‘BA29’ quince, genes encoding ammonium transporters (AMT1) expressed in the roots were identified under no N supply, and their diversity and regulation upon N application after fruit set was investigated. In this study, thin roots of ‘Rocha’pear/’BA29’ quince were sampled in a plot without N fertilization and four disti...
In order to identify genes that are specifically regulated during the expression of the resistance mechanism of the apple cv. Florina towards the apple scab fungus, the genetic expression pattern in plants inoculated with a conidial suspension of Venturia inaequalis was compared with the expression pattern in plants inoculated with water, by differential display RT-PCR. Amplified products derived from the combi...
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