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Golgi apparatus dynamics during mouse oocyte in vitro maturation: effect of the...
Moreno, Ricardo D.; Schatten, Gerald; Ramalho-Santos, JoãoWe have studied Golgi apparatus dynamics during mouse oocyte in vitro maturation, employing both live imaging with the fluorescent lipid BODIPY-ceramide and immunocytochemistry using several specific markers (beta-COP, giantin, and TGN38). In germinal vesicle oocytes the Golgi consisted of a series of structures, possibly cisternal stacks, dispersed in the ooplasm, but slightly more concentrated in the interior...
Membrane Trafficking Machinery Components Associated with the Mammalian Acrosom...
Ramalho-Santos, João; Moreno, Ricardo D.; Wessel, Gary M.; Chan, Edward K. L.; Schatten, GeraldActive trafficking from the Golgi apparatus is involved in acrosome formation, both by delivering acrosomal contents to the nascent secretory vesicle and by controlling organelle growth and shaping. During murine spermiogenesis, Golgi antigens (giantin, [beta]-COP, golgin 97, mannosidase II) are detected in the acrosome until the late cap-phase spermatids, but are not found in testicular spermatozoa (maturation...
SNAREs in Mammalian Sperm: Possible Implications for Fertilization
Ramalho-Santos, João; Moreno, Ricardo D.; Sutovsky, Peter; Chan, Anthony Wing-Sang; Hewitson, Laura; Wessel, Gary M.; Simerly, Calvin R.Soluble N-ethylmalameide-sensitive factor attachment protein receptor (SNARE) proteins are present in mammalian sperm and could be involved in critical membrane fusion events during fertilization, namely the acrosome reaction. Vesicle-associated membrane protein/synaptobrevin, a SNARE on the membrane of a vesicular carrier, and syntaxin 1, a SNARE on the target membrane, as well as the calcium sensor synaptotag...
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