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Controlling the Morphology in DNA Condensation and Precipitation
Pinto, Maria F. V.; Morán, M. Carmen; Miguel, M. Graça; Lindman, Björn; Jurado, Amália S.; Pais, Alberto A. C. C.This work addresses the influence of solution inhomogeneity on conformation, aggregation, and coil/globule and bundle/single chain coexistence of T4 DNA molecules. The inhomogeneity is induced by mixing two solutions containing, respectively, protamine and DNA, with different relative concentrations, but aiming at producing the same final concentrations. The study was conducted by means of fluorescence microsco...
Mixed Protein Carriers for Modulating DNA Release
Morán, M. Carmen; Pais, Alberto A. C. C.; Ramalho, Amilcar; Miguel, M. Graça; Lindman, BjörnAqueous mixtures of oppositely charged polyelectrolytes undergo associative phase separation, resulting in coacervation, gelation, or precipitation. This phenomenon has been exploited in forming DNA gel particles by interfacial diffusion. We report here the formation of DNA gel particles by mixing solutions of double-stranded DNA with aqueous solutions containing two cationic proteins, lysozyme and protamine su...
Cationic agents for DNA compaction
Gaweda, Sylwia; Morán, M. Carmen; Pais, Alberto A. C. C.; Dias, Rita S.; Schillén, Karin; Lindman, Björn; Miguel, M. GraçaFluorescence microscopy was used to investigate the conformational changes of individual T4 DNA molecules induced by different compacting agents, namely the cationic surfactants, cetyltrimethylammonium bromide (CTAB) and chloride (CTAC), iron(III), lysozyme, and protamine sulfate. A protocol for establishing size estimates is suggested to obtain reproducible results. Observations show that in the presence of ly...
Surfactant−DNA Gel Particles: Formation and Release Characteristics
Morán, M. Carmen; Miguel, M. Graça; Lindman, BjörnAqueous mixtures of oppositely charged polyelectrolytes undergo associative phase separation, resulting in coacervation, gelation, or precipitation. This phenomenon has been exploited here to form DNA gel particles by interfacial diffusion. We report on the formation of DNA gel particles by mixing solutions of DNA (either single-stranded (ssDNA) or double-stranded (dsDNA)) with solutions of cationic surfactant ...
DNA Gel Particles: Particle Preparation and Release Characteristics
Morán, M. Carmen; Miguel, M. Graça; Lindman, BjörnAqueous mixtures of oppositely charged polyelectrolytes undergo associative phase separation, resulting in coacervation, gelation, or precipitation. This phenomenon has been exploited here to form DNA gel particles by interfacial diffusion. We report the formation of DNA gel particles by mixing solutions of DNA (either single-stranded (ssDNA) or double-stranded (dsDNA)) with solutions of cationic surfactant CTA...
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