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State and specific growth estimation in heterologous protein production by Pich...
Barrig??n, J. M.; Ramon, R.; Rocha, I.; Valero, F.; Ferreira, E. C.; Montesinos, J. L.The productivity of an industrial fermentation process involving a filamentous microbe is heavily dependent on the morphological form adopted by the organism. The development of systems capable of rapidly and accurately characterizing morphology within a given process represents a significant challenge, as the complex phenotypes that are manifested are not easily quantified. Conventional parameters employed in ...
Observer based estimation in heterologous protein production by Pichia pastoris
Barrigón, J. M.; Rocha, I.; Valero, F.; Ferreira, E. C.; Montesinos, J. L.The methylotrophic yeast Pichia pastoris has been widely reported as a suitable expression system for both basic research and industrial use. In order to achieve high productivity, constant product quality, as well as to allow optimization and control of biotechnological processes, real-time monitoring of the key fermentation variables (biomass, substrate and products) is of major academic and industrial releva...
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