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Silver colloidal nanoparticles : effect on matrix composition and structure of ...
Monteiro, D. R.; Silva, Sónia Carina; Negri, M.; Gorup, L. F.; Camargo, E. R.; Oliveira, Rosário; Barbosa, D. B.; Henriques, MarianaAim : The aim of this study was to assess the effect of different silver nanoparticles (SN) concentrations on the matrix composition and structure of Candida albicans and Candida glabrata biofilms. Methods and Results : Candida biofilms were developed in 6-well microtiter plates during 48 h. After, these biofilms were exposed to 13·5 or 54 μg SN ml−1 for 24 h. Then, extracellular matrices were extracted from bi...
Silver nanoparticles : influence of stabilizing agent and diameter on antifunga...
Monteiro, D. R.; Silva, Sónia Carina; Negri, M.; Gorup, L. F.; Camargo, E. R.; Oliveira, Rosário; Barbosa, D. B.; Henriques, MarianaAim : The purpose of this work was to evaluate the size-dependent antifungal activity of different silver nanoparticles (SN) colloidal suspensions against Candida albicans and Candida glabrata mature biofilms. Methods and Results: The research presented herein used SN of three different average sizes (5, 10 and 60 nm), which were synthesized by the reduction of silver nitrate through sodium citrate and which w...
Effect of pretreatment of Candida biofilm cells with silver nanoparticles on th...
Monteiro, D. R.; Negri, M.; Silva, Sónia Carina; Camargo, E. R.; Gorup, L. F.; Oliveira, Rosário; Barbosa, D.; Henriques, MarianaObjectives: The pathogenicity of Candida spp. has been recognized by some factors, including adhesion to host cells or inert surfaces and biofilm formation. In addition, the dispersal of biofilm cells may act as a continuous source of infection. Thus, the aim of this study was to evaluate the adhesion capacity of Candida albicans and Candida glabrata cells recovered from Candida biofilms treated with silver nan...
Virulence factors of non-Candida albicans Candida species
Silva, Sónia Carina; Negri, M.; Monteiro, D. R.; Henriques, Mariana; Oliveira, Rosário; Azeredo, JoanaInfections caused by Candida species (candidosis) have greatly increased over recent years, mainly due to the escalation of the AIDS epidemic, population ageing, increasing number of immunocompromised patients and the more widespread use of indwelling medical devices. Besides Candida albicans, non-Candida albicans Candida (NCAC) species such as Candida glabrata, Candida tropicalis and Candida parapsilosis are n...
Silver colloidal nanoparticles: antifungal effect against Candida albicans and ...
Monteiro, D. R.; Gorup, L. F.; Silva, Sónia Carina; Negri, M.; Camargo, E. R.; Oliveira, Rosário; Barbosa, D.; Henriques, MarianaThe aim of this study was to evaluate the effect of silver nanoparticles (SN) against Candida albicans and Candida glabrata adhered cells and biofilms. SN (average diameter 5 nm) were synthesized by silver nitrate reduction with sodium citrate and stabilized with ammonia. Minimal inhibitory concentration (MIC) tests were performed for C. albicans (n = 2) and C. glabrata (n = 2) grown in suspension following the...
Effect of silver nanoparticles against Candida albicans and Candida glabrata bi...
Monteiro, D. R.; Silva, Sónia Carina; Negri, M.; Camargo, E. R.; Oliveira, Rosário; Henriques, Mariana; Barbosa, D. B.Objectives: Fungal infections in immunocompromised patients have been contributing to the increasing morbidity and mortality of these patients, especially associated to yeast resistance to antifungal therapy. The increase in antibiotic-resistant microorganisms has prompted interest in the use of silver as an antimicrobial agent. Thus, the aim of this study was to evaluate the antifungal efficacy of silver nanop...
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