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Purification, Characterization and Partial Amino Acid Sequencing of Two New Asp...
Veríssimo, Paula; Faro, Carlos; Moir, Arthur J. G.; Lin, Yingzhang; Tang, Jordan; Pires, EuclidesTwo new aspartic proteinases have been isolated from stigmas of the cardoon Cynara cardunculus L. by a two-step purification procedure including extraction at low pH, gel filtration on Superdex 200, and ion-exchange chromatography on Mono Q. To follow the conventional nomenclature for aspartic proteinases, we have named these proteinases cardosin A and cardosin B. On SDS/PAGE, cardosin A migrated as two bands w...
Data: 1996
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Origem: Estudo Geral - Universidade de Coimbra
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