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Traumatismo Cervical Perfurante Caso Clínico

Os autores  apresentam o caso clínico de uma criança de 4 anos que após traumatismo cervical perfurante» desenvolveu laceração da traqueia cervical com quadro grave de pneumomediastino. Foi submetido a tratamento cirúrgico, tendo tido evolução clínica favorável, mantendo-se sempre em ventilação espontânea. Teve alta ao 1° dia de pós-operatório, clinicamente estável.A propósito deste caso faz-se uma revisão da a...

A new approach to bacterial colony morphotyping by matrix-assisted laser desorp...

Matrix assisted laser desorption ionization time of flight mass spectrometry has been explored as a tool to bacterial colony morphotyping.To this end,four colony morphotypes of Pseudomonas aeruginosa and four of Staphylococcus aureus were analysed using intact bacteria.Results suggest that mass spectrometry of intact bacteria could,in some extent,be used to complement the classical morphological classification ...

Standard methods for molecular research in Apis mellifera

From studies of behaviour, chemical communication, genomics and developmental biology, among many others, honey bees have long been a key organism for fundamental breakthroughs in biology. With a genome sequence in hand, and much improved genetic tools, honey bees are now an even more appealing target for answering the major questions of evolutionary biology, population structure, and social organization. At th...

Differential effects of methoxyamine on doxorubicin cytotoxicity and genotoxici...

Pharmacological inhibition of DNA repair is a promising approach to increase the effectiveness of anticancer drugs. The chemotherapeutic drug doxorubicin (Dox) may act, in part, by causing oxidative DNA damage. The base excision repair (BER) pathway effects the repair of many DNA lesions induced by reactive oxygen species (ROS). Methoxyamine (MX) is an indirect inhibitor of apurinic/apyrimidinic endonuclease 1 ...

Synthesis and Biological Activity of 6-Selenocaffeine: Potential Modulator of C...

We report the development of a new microwave-based synthetic methodology mediated by Woollins’ reagent that allowed an efficient conversion of caffeine into 6-selenocaffeine. A preliminary evaluation on the modulation of antioxidant activity upon selenation of caffeine, using the DPPH assay, indicated a mild antioxidant activity for 6-selenocaffeine, contrasting with caffeine, that exhibited no antioxidant acti...

Microalgae as a sustainable raw material for biofuels production and high added...

Pre-treatment optimization of Scenedesmus obliquus microalga for bioethanol pro...

The present work deals with the optimization of cellular disruption and sugar extraction from the microalgae Scenedesmusobliquus (Sc) for bioethanol production. Among the physical and physicochemical methods tested, the best results were obtained with acid hydrolysis by H2SO4 (2 N), at 120 °C for 30 min and using dried biomass. The sugar extraction efficiency level reached was 95.6% when compared to the harsh q...

Bioethanol production from Scenedesmus obliquus sugars: the influence of photob...

A closed-loop vertical tubular photobioreactor (PBR), specially designed to operate under conditions of scarce flat land availability and irregular solar irradiance conditions, was used to study the potential of Scenedesmus obliquus biomass/sugar production. The results obtained were compared to those from an open-raceway pond and a closed-bubble column. The influence of the type of light source and the regime ...

Cytotoxic effects of cadmium in mammary epithelial cells: Protective role of th...

Human exposure to cadmium (Cd) occurs via different routes, including diet. The increasing amount of data linking Cd with different cellular effects in the mammary gland justifies additional toxicological assessments using human mammary epithelial cells. This work aimed therefore to assess the cytotoxic effects of Cd in MCF10A cells and to characterize the cytoprotective role of the macrocycle [15]pyN5 in the f...

Colite por Clostridium difficile numa enfermaria de medicina interna

A colite por Clostridium difficile condiciona cada vez mais morbilidade e mortalidade em doentes hospitalizados. Estudaram-se retrospectivamente características epidemiológicas e clínicas dos casos de colite por Clostridium difficile ocorridos numa enfermaria de Medicina Interna, num certo período de tempo, como contributo à melhoria da abordagem desta infecção em Portugal. A incidência da doença esteve ao níve...