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Laccase production by free and immobilized mycelia of Peniophora cinerea and Tr...
Silvério, Sara C.; Moreira, Sérgio; Milagres, Adriane M. F.; Macedo, Eugénia A.; Teixeira, J. A.; Mussatto, Solange I.The production of laccase by immobilized mycelia of Peniophora cinerea and Trametes versicolor was studied. In an initial stage, experimental assays were performed in Erlenmeyer flasks using free and immobilized mycelium, and the performance of the fungal strains to produce the enzyme was compared. Both fungi adhered into the support material (a synthetic fiber), growing not only on the surface but also in the ...
Decolorization of salt-alkaline effluent with industrial reactive dyes by lacca...
Moreira Neto, S. L.; Mussatto, Solange I.; Machado, K. M. G.; Milagres, Adriane M. F.The discharge of highly coloured synthetic dye effluents into rivers and lakes is harmful to the water bodies, and therefore, intensive researches have been focussed on the decolorization of wastewater by biological, physical or chemical treatments. In the present study, 12 basidiomycetes strains from the genus Pleurotus, Trametes, Lentinus, Peniophora, Pycnoporus, Rigidoporus, Hygrocybe and Psilocybe were eval...
Análise do perfil de adsorção das enzimas envolvidas na sacarificação enzimátic...
Monte, J. R.; Milagres, Adriane M. F.; Mussatto, Solange I.A produção de etanol de segunda geração é uma realidade brasileira que já vem sendo implementada na indústria. Porém, vários fatores que governam a sacarificação enzimática de materiais lignocelulósicos ainda necessitam ser melhor esclarecidos, de forma a baixar o custo e aumentar o rendimento final de monossacarídeos. A adsorção das enzimas durante a sacarificação da celulose é um fator determinante para uma h...
Recovery of Peniophora cinerea laccase using aqueous two-phase systems composed...
Moreira, Sérgio; Silvério, Sara C.; Macedo, Eugénia A.; Milagres, Adriane M. F.; Teixeira, J. A.; Mussatto, Solange I.Aqueous two-phase systems (ATPSs) composed by UCON (ethylene oxide/propylene oxide copolymer) and potassium phosphate salts were for the first time evaluated in the recovery of Peniophora cinerea laccase from complex fermented medium. The ATPSs were obtained by combining the random copolymer UCON with KH2PO4, potassium phosphate buffer pH 7 or K2HPO4. According to the results, protein partition occurred predomi...
Interference of some ATPS phase-forming components in protein determination by ...
Silvério, Sara C.; Moreira, Sérgio; Milagres, Adriane M. F.; Macedo, Eugénia A.; Teixeira, J. A.; Mussatto, Solange I.The interference of some specific aqueous two-phase system (ATPS) phase-forming components in bovine serum albumin (BSA) determination by the Bradford method was investigated. For this purpose, calibration curves were obtained for BSA in the presence of different concentrations of salts and polymers. A total of 19 salts [Na(2)SO(4), (NH(4))(2)SO(4), MgSO(4), LiSO(4), Na(2)HPO(4), sodium phosphate buffer (pH 7.0...
Laccase production by free and immobilized fungal mycelium of Trametes versicolor
Silvério, Sara; Moreira, Sérgio; Mussatto, Solange I.; Milagres, Adriane M. F.; Macedo, Eugénia; Teixeira, J. A.The production of laccase by free and immobilized mycelium of Trametes versicolor was evaluated. Fermentation experiments were carried out using a Trametes defined medium [1] supplemented with tween-80 (0.5%, w/v) and xylidine (30 μM) to stimulate and induce the secretion of extracellular enzyme. The support for the mycelium immobilization consisted in 0.3 cm3 cubes of synthetic fiber (Scotch Brite, 3M Spain, S...
Relevância de compostos de baixa massa molar produzidos por fungos e envolvidos...
Arantes,Valdeir; Milagres,Adriane M. F.Over the last decade, evidences have been shown that the wood biodegradation by fungi is not only a result of the action of their enzymatic machinery but also of various low molecular weight non-enzymatic compounds, especially in fungi that promote brown and white decay, which in nature are the major wood decaying microorganisms. The present review focuses on the recent theories involving these low molecular we...
The effect of agitation speed, enzyme loading and substrate concentration on en...
Mussatto, Solange I.; Dragone, Giuliano; Fernandes, Marcela; Milagres, Adriane M. F.; Roberto, Inês ConceiçãoBrewer’s spent grain components (cellulose, hemicellulose and lignin) were fractionated in a two-step chemical pretreatment process using dilute sulfuric acid and sodium hydroxide solutions. The cellulose pulp produced was hydrolyzed with a cellulolytic complex, Celluclast 1.5 L, at 45 ºC to convert the cellulose into glucose. Several conditions were examined: agitation speed (100, 150 and 200 rpm), enzyme load...
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