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Proteinaceous microspheres for targeted RNA delivery prepared by an ultrasonic ...
Shimanovich, Ulyana; Eliaz, Dror; Zigdon, Sally; Volkov, Vadim; Aizer, Adva; Paulo, Artur Cavaco; Michaeli, S.; Shav-Tal, Yaron; Gedanken, AharonIn the present work we used sonochemically prepared proteinaceous BSA spheres as a novel RNA-delivery system. The preparation of RNA-loaded BSA spheres was accomplished using an environmental friendly method termed the “ultrasonic emulsification method”. It was demonstrated that ultrasonic waves do not cause the RNA chains to degrade and the RNA molecules remain untouched. The BSA–RNA complex was successfully i...
Encapsulation of RNA molecules in BSA microspheres and internalization into Try...
Shimanovich, Ulyana; Tkacz, Itai Dov; Eliaz, Dror; Paulo, Artur Cavaco; Michaeli, S.; Gedanken, AharonRNA was encapsulated in bovine serum albumin (BSA) microspheres using a one-step sonochemical process from an water–oil solvent biphasic system. Confocal microcoscopy and fluorescence-activated cell sorting indicate that a CY3-RNA (RNA labeled with red fluorescent indocarbocyanine Cy3 dye) sphere is encapsulated in the BSA outer sphere. The diameter of the sphere depends on the number of nucleotides of the RNA,...
Microspheres of mixed proteins
Angel, U.; Matas, D.; Michaeli, S.; Paulo, Artur Cavaco; Gedanken, AharonThis paper describes the synthesis of mixed proteinaceous microspheres (MPMs) by the sonochemical method. The current fundamental research follows the research of Suslick and co-workers who have developed a method by which high-intensity ultrasound is used to make aqueous suspensions of proteinaceous microcapsules filled with water-insoluble liquids.1 By using high-intensity ultrasound, we have synthesized micr...
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