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Automatic identification of activated sludge disturbances and assessment of ope...
Amaral, A. L.; Mesquita, D. P.; Ferreira, E. C.Activated sludge systems are prone to be affected by changes in operating conditions leading to problems such as pinpoint flocs formation, filamentous bulking, dispersed growth, and viscous bulking. These problems are often related with the floc structure and filamentous bacteria contents. In this work, a lab-scale activated sludge system was operated sequentially obtaining filamentous bulking, pinpoint floc fo...
Prediction of intracellular storage polymers using quantitative image analysis ...
Mesquita, D. P.; Leal, C.; Cunha, J. R.; Oehmen, Adrian; Amaral, A. L.; Reis, Maria A. M.; Ferreira, E. C.The present study focuses on predicting the concentration of intracellular storage polymers in enhanced biological phosphorus removal (EBPR) systems. For that purpose, quantitative image analysis techniques were developed for determining the intracellular concentrations of PHA (PHB and PHV) with Nile blue and glycogen with aniline blue staining. Partial least squares (PLS) were used to predict the standard anal...
Development of image analysis methodologies to quantify intracellular PHA, poly...
Amaral, A. L.; Leal, C. S.; Mesquita, D. P.; Cunha, J. R.; Oehmen, A.; Reis, M. A. M.; Ferreira, E. C.In wastewater treatment plants (WWTP), enhanced biological phosphorus removal (EBPR) processes are performed by mixed cultures containing polyphosphate (PAO) and glycogen accumulating organisms (GAO). In these processes, it is of crucial importance to monitor the intracellular metabolism, namely glycogen, polyhydroxyalkanoates (PHA) and polyphosphate (polyP) inclusions, to determine its efficiency. However, tra...
Image analysis for automatic characterization of polyhydroxyalcanoates granules
Mesquita, D. P.; Selvaggio, Gianluca; Cunha, J. R.; Leal, Cristiano S.; Amaral, A. L.; Ferreira, E. C.A new monitoring approach for polyhydroxyalcanoates (PHA) granules identification and characterization based on image analysis procedures is proposed. PHA granules were analyzed by Sudan Black B (SBB) staining in an enhanced biological phosphorus removal (EBPR) system. Color images captured on an optical microscope were analyzed through quantitative image analysis. The distribution of PHA granules was estimated...
Quantitative image analysis for the characterization of microbial aggregates in...
Costa, J. C.; Mesquita, D. P.; Amaral, A. L.; Alves, M. M.; Ferreira, E. C.Quantitative image analysis techniques have gained an undeniable role in several fields of research during the last decade. In the field of biological wastewater treatment (WWT) processes, several computer applications have been developed for monitoring microbial entities, either as individual cells or in different types of aggregates. New descriptors have been defined that are more reliable, objective, and use...
Novel method to quantify intracellular accumulation of polyphosphate in EBPR sy...
Mesquita, D. P.; Amaral, A. L.; Leal, Cristiano; Carvalheira, Mónica; Cunha, J. R.; Oehmen, Adrian; Reis, Maria A. M.; Ferreira, E. C.A new method for intracellular storage polyphosphate (poly-P)identification and quantification in enhanced biological phosphorus removal (EBPR) systems is proposed based on image analysis. In EBPR systems, 4',6-diamidino-2-phenylindole (DAPI) is usually combined with fluororescent in situ hybridization (FISH) to evaluate the microbial community. The proposed technique is based on an image analysis procedure spe...
Activated sludge characterization through microscopy : a review on quantitative...
Mesquita, D. P.; Amaral, A. L.; Ferreira, E. C.In wastewater treatment processes, and particularly in activated sludge systems, efficiency is quite dependent on the operating conditions, and a number of problems may arise due to sludge structure and proliferation of specific microorganisms. In fact, bacterial communities and protozoa identification by microscopy inspection is already routinely employed in a considerable number of cases. Furthermore, quantit...
Biopolymer monitoring using quantitative image analysis techniques
Carvalheira, Mónica; Mesquita, D. P.; Leal, Cristiano; Oehmen, Adrian; Carvalho, Gilda; Cunha, J. R.; Amaral, A. L.; Ferreira, E. C.; Reis, Maria A. M.Polyhydroxyalkanoates (PHAs) are intracellular biopolymers with many applications, particularly as substitutes of polypropylene and polyethylene, due to their thermoplastic properties and biocompatible nature. Furthermore, glycogen is a polysaccharide of glucose with high importance in the metabolism of microbial communities and polyphosphate is a microbial storage compound that should be recovered in order to ...
Intracellular poly-P assessment by DAPI staining and image analysis
Amaral, A. L.; Mesquita, D. P.; Leal, C.; Carvalheira, Mónica; Oehmen, Adrian; Reis, Maria A. M.; Ferreira, E. C.In wastewater treatment, enhanced biological phosphorus removal (EBPR) is considered a well-established process to remove phosphate (P). EBPR is based on the activity of polyphosphate-accumulating organisms (PAOs) able to take up and store large amounts of P as intracellular (poly-P) granules. However, monitoring poly-P in mixed cultures is usually performed by a laborious and time consuming off-line chemical a...
Study of chemical oxygen demand and ammonia removal efficiencies by image analy...
Amaral, A. L.; Mesquita, D. P.; Ferreira, E. C.Activated sludge systems are frequently used in wastewater treatment for chemical oxygen demand (COD) and ammonia removal. However, several problems can affect the operation of these systems leading to abnormal conditions such as filamentous bulking, viscous bulking and pinpoint flocs, among others. These occurrences, which may lead to the decrease of COD and ammonia removal efficiencies, are linked to biomass ...
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