Microcystins (MCs) are toxins produced by cyanobacteria from water environments that can induce acute and chronic effects on humans and animals, after ingestion/contact with contaminated water. This group of cyclic heptapeptides comprises approximately 80 variants, being microcystin-LR (MCLR) the most frequent and toxic variant. The studies of MCs effects on cell lines often lead to contradictory results, given...
In Portugal, potentially toxic cyanobacteria from Planktothrix genus have become frequently observed in freshwater reservoirs. Identification of Planktothrix species through optical microscopy is complicated due to limited morphological differences among them. The aim of this work was to determine the most suitable phylogenetic markers that could be used for the molecular identification of Planktothrix species....
A microcistina-LR (MCLR) é amplamente reconhecida pela sua hepatotoxicidade. No entanto sabe-se que também afecta outros orgãos tais como o cérebro, rins e intestinos. Este trabalho tem como objectivo comparar os efeitos tóxicos da exposição a uma gama de concentrações de MCLR pura e de extractos de cianobactérias em linhas celulares hepáticas, renais e de intestino, representativas dos orgãos de acumulação da ...
A coleção de culturas de algas Estela Sousa e Silva (ESSACC) foi criada em 1956 e reside atualmente no Laboratório de Biologia e Ecotoxicologia no Instituto Nacional de Saúde Dr. Ricardo Jorge. A ESSACC foi implementada em resposta à necessidade de um repositório de material biológico para investigação na área do fitoplâncton. Este importante recurso biológico contém culturas monoclonais de algas eucarióticas e...
Microcystin-LR (MCLR), a potent hepatotoxin, is transported selectively into the cells throught specific membrane polypeptides mostly present in the liver. These transporters are also expressed in the brain, kidneys and intestine, although the toxicity of MCLR in these cell types is still poorly understood. In this study, morphological, ultrastructural and biochemical analyses were performed in hepatic, renal a...
Microcystin-LR (MCLR) is a potent hepatotoxin produced by freshwater cyanobacteria, being responsible for acute and chronic hazards to animal and human health. Despite the increasing recognition of its toxic effects, the cellular basis of MCLR-induced toxicity is still poorly understood. In this work, morphological, ultrastructural and biochemical (MTT and Neutral Red-NR) analyses were performed to evaluate the...
Dissertação de Mestrado em Biologia Humana e Ambiente apresentada à Faculdade de Ciências da Universidade de Lisboa, 2009 ; Tese orientada por: Professora Doutora Ana Amorim (Professora Auxiliar da Faculdade de Ciências da Universidade de Lisboa); Doutora Elsa Alverca (Investigadora do Laboratório de Biologia e Ecotoxicologia, Departamento de Saúde Ambiental,Instituto Nacional de Saúde Doutor Ricardo Jorge) ; ...
Tese de mestrado em Biologia (Biologia Humana e Ambiente), Universidade de Lisboa, Faculdade de Ciências ; Microcystin-LR (MCLR) is a natural occurring freshwater cyanotoxin, recognized as one of the most toxic microcystin variants. It is thought to be responsible for cases of livestock and human intoxication due to consumption of toxic cyanobacteria-contaminated water. Although considered a hepatotoxin, MCLR ...
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