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Physicochemical and functional characterization of a biosurfactant produced by ...

Rodrigues, L. R.; Teixeira, J. A.; Mei, Henny van der; Oliveira, Rosário

Isolation and identification of key components of the crude biosurfactant produced by Lactococcus lactis 53 was studied. Fractionation was achieved by hydrophobic interaction chromatography which allowed the isolation of a fraction rich in glycoproteins. Molecular (by Fourier transform infrared spectroscopy) and elemental compositions (by X-ray photoelectron spectroscopy) were determined. Critical micelle conce...


Isolation and partial characterization of a biosurfactant produced by streptoco...

Rodrigues, L. R.; Teixeira, J. A.; Mei, Henny van der; Oliveira, Rosário

Isolation and characterization of the surface active components from the crude biosurfactant produced by Streptococcus thermophilus A was studied. A fraction rich in glycolipids was obtained by the fractionation of crude biosurfactant using hydrophobic interaction chromatography. Molecular (by Fourier transform infrared spectroscopy) and elemental compositions (by X-ray photoelectron spectroscopy) were determin...


Response surface optimization of the medium components for the production of bi...

Rodrigues, L. R.; Teixeira, J. A.; Oliveira, Rosário; Mei, Henny van der

Optimization of the medium for biosurfactants production by probiotic bacteria (Lactococcus lactis 53 and Streptococcus thermophilus A) was carried out using response surface methodology. Both biosurfactants were proved to be growth-associated, thus the desired response selected for the optimization was the biomass concentration. The selected factors based on MRS medium for L. lactis 53 growth were peptone, mea...


Inhibition of microbial adhesion to silicone rubber treated with biosurfactant ...

Rodrigues, L. R.; Mei, Henny van der; Banat, Ibrahim M.; Teixeira, J. A.; Oliveira, Rosário

Prova tipográfica (In Press) ; Microbial adhesion of four bacterial and two yeast strains isolated from explanted voice prostheses to silicone rubber before and after conditioning with a biosurfactant obtained from the probiotic bacterium Streptococcus thermophilus A was investigated in a parallel plate flow chamber. The silicone rubber with and without an adsorbed biosurfactant layer was characterized using c...


Interference in adhesion of bacteria and yeasts isolated from explanted voice p...

Rodrigues, L. R.; Banat, Ibrahim M.; Mei, Henny van der; Teixeira, J. A.; Oliveira, Rosário

Aims: The effects and extent of adhesion of four different bacterial and two yeast strains isolated from explanted voice prostheses to silicone rubber with and without an adsorbed rhamnolipid biosurfactant layer obtained from Pseudomonas aeruginosa DS10-129 was studied. Methods and Results: The ability of rhamnolipid biosurfactant to inhibit adhesion of micro-organisms to silicone rubber was investigated in a p...


Influence of biosurfactants from probiotic bacteria on formation of biofilms on...

Rodrigues, L. R.; Mei, Henny van der; Teixeira, J. A.; Oliveira, Rosário

Biofilms were grown on preconditioned voice prostheses with biosurfactants obtained from probiotic bacteria Lactococcus lactis 53 and Streptococcus thermophilus A in an artificial throat model. Both biosurfactants greatly reduced microbial numbers on prostheses and also induced a decrease in the airflow resistance that occurs on voice prostheses after biofilm formation. This study presents a promising strategy ...


Biosurfactant from Lactococcus lactis 53 inhibits microbial adhesion on silicon...

Rodrigues, L. R.; Mei, Henny van der; Teixeira, J. A.; Oliveira, Rosário

The ability of biosurfactant obtained from the probiotic bacterium Lactococcus lactis 53 to inhibit adhesion of four bacterial and two yeast strains isolated from explanted voice prostheses to silicone rubber with and without an adsorbed biosurfactant layer was investigated in a parallel-plate flow chamber. The microbial cell surfaces and the silicone rubber with and without an adsorbed biosurfactant layer were...


The influence of biosurfactants from probiotic bacteria on the formation of voi...

Rodrigues, L. R.; Mei, Henny van der; Teixeira, J. A.; Oliveira, Rosário


Biosurfactant from Streptococcus thermophilus A inhibit microbial adhesion on s...

Rodrigues, L. R.; Mei, Henny van der; Teixeira, J. A.; Oliveira, Rosário

The ability of the biosurfactant obtained from Streptococcus thermophilus A to inhibit adhesion of four bacterial and two yeast strains isolated from explanted voice prostheses to silicone rubber with and without an adsorbed biosurfactant layer was investigated in a parallel-plate flow chamber. The microbial cell surfaces and the silicone rubber with and without an adsorbed biosurfactant layer were characterize...


Biosurfactant from Lactococcus lactis 53 inhibits microbial adhesion on silicon...

Rodrigues, L. R.; Mei, Henny van der; Teixeira, J. A.; Oliveira, Rosário


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    Financiadores do RCAAP

Fundação para a Ciência e a Tecnologia Universidade do Minho   Governo Português Ministério da Educação e Ciência Programa Operacional da Sociedade do Conhecimento União Europeia