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Spatiotemporal regulation of mitotic spindle dynamics and its contribution for ...
Matos, Irina Alexandra Cardoso deBiomedicina ; Doctoral Programme in Biomedicine
Drosophila S2 cells as a model system to investigate mitotic spindle dynamics, ...
Moutinho-Pereira, Sara; Matos, Irina; Maiato, HelderIn order to perpetuate their genetic content, eukaryotic cells have developed a microtubule-based machine known as the mitotic spindle. Independently of the system studied, mitotic spindles share at least one common characteristic – the dynamic nature of microtubules. This property allows the constant plasticity needed to assemble a bipolar structure, make proper kinetochoremicrotubule attachments, segregate ch...
Synchronizing chromosome segregation by flux-dependent force equalization at ki...
Matos, Irina; Pereira, António J.; Lince-Faria, Mariana; Cameron, Lisa A.; Salmon, Edward D.; Maiato, HelderThe synchronous movement of chromosomes during anaphase ensures their correct inheritance in every cell division. This reflects the uniformity of spindle forces acting on chromosomes and their simultaneous entry into anaphase. Although anaphase onset is controlled by the spindle assembly checkpoint, it remains unknown how spindle forces are uniformly distributed among different chromosomes. In this paper, we sh...
Dynein and Mast/Orbit/CLASP have antagonistic roles in regulating kinetochore-m...
Reis, Rita; Feijão, Tália; Gouveia, Susana; Pereira, António J.; Matos, Irina; Sampaio, Paula; Maiato, Helder; Sunkel, Claudio E.Establishment and maintenance of the mitotic spindle requires the balanced activity of microtubule-associated proteins and motors. In this study we have addressed how the microtubule plus-end tracking protein Mast/Orbit/CLASP and cytoplasmic dynein regulate this process in Drosophila melanogaster embryos and S2 cells. We show that Mast accumulates at kinetochores early in mitosis, which is followed by a polewar...
Dissecting mitosis with laser microsurgery and RNAi in Drosophila cells
Pereira, António J.; Matos, Irina; Lince-Faria, Mariana; Maiato, HelderProgress from our present understanding of the mechanisms behind mitosis has been compromised by the fact that model systems that were ideal for molecular and genetic studies (such as yeasts, C. elegans or Drosophila) were not suitable for intracellular micromanipulation. Unfortunately, those systems that were appropriate for micromanipulation (like newt lung cells, PtK1 cells or insect spermatocytes) are not a...
Tissue microarrays for testing basal biomarkers in familial breast cancer cases
Dufloth,Rozany Mucha; Matos,Irina; Schmitt,Fernando; Zeferino,Luiz CarlosCONTEXT AND OBJECTIVE: The proteins p63, p-cadherin and CK5 are consistently expressed by the basal and myoepithelial cells of the breast, although their expression in sporadic and familial breast cancer cases has yet to be fully defined. The aim here was to study the basal immunopro-file of a breast cancer case series using tissue microarray technology. DESIGN AND SETTING: This was a cross-sectional study at U...
Mammalian CLASP1 and CLASP2 cooperate to ensure mitotic fidelity by regulating ...
Pereira, Ana L.; Pereira, António J.; Maia, Ana R.R.; Drabek, Ksenija; Sayas, C. Laura; Hergert, Polla J.; Lince-Faria, Mariana; Matos, IrinaCLASPs are widely conserved microtubule plus-end–tracking proteins with essential roles in the local regulation of microtubule dynamics. In yeast, Drosophila, and Xenopus, a single CLASP orthologue is present, which is required for mitotic spindle assembly by regulating microtubule dynamics at the kinetochore. In mammals, however, only CLASP1 has been directly implicated in cell division, despite the existence ...
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