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A ClpB Chaperone Knockout Mutant of Mesorhizobium ciceri Shows a Delay in the R...

Brígido, Clarisse; Robledo, Marta; Menéndez, Esther; Mateos, Pedro F.; Oliveira, Solange

Several molecular chaperones are known to be involved in bacteria stress response. To investigate the role of chaperone ClpB in rhizobia stress tolerance as well as in the rhizobiaplant symbiosis process, the clpB gene from a chickpea microsymbiont, strain Mesorhizobium ciceri LMS-1, was identified and a knockout mutant was obtained. The ClpB knockout mutant was tested to several abiotic stresses, showing that ...


Strains of Mesorhizobium amorphae and M. tianshanense carrying symbiotic genes ...

Rivas, Raul; Laranjo, Marta; Mateos, Pedro; Oliveira, Solange; Martínez-Molina, Eustoquio; Velázquez, Encarna

Aims: To identify several strains of Mesorhizobium amorphae and Mesorhizobium tianshanense nodulating Cicer arietinum in Spain and Portugal, and to study the symbiotic genes carried by these strains. Methods and Results: The sequences of 16S-23S intergenic spacer (ITS), 16S rRNA gene and symbiotic genes nodC and nifH were analysed. According to their 16S rRNA gene and ITS sequences, the strains from this study ...


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Fundação para a Ciência e a Tecnologia Universidade do Minho   Governo Português Ministério da Educação e Ciência Programa Operacional da Sociedade do Conhecimento União Europeia