Encontrados 21 documentos, a visualizar página 1 de 3
Odorant binding proteins : a biotechnological tool for odour control
Silva, Carla Manuela; Matamá, Maria Teresa; Azoia, Nuno G.; Mansilha, Catarina; Casal, Margarida; Paulo, Artur CavacoThe application of an odorant binding protein for odour control and fragrance delayed release from a textile surface was first explored in this work. Pig OBP-1 gene was cloned and expressed in Escherichia coli , and the purified protein was biochemically characterized. The IC50 values(concentrations of competitor that caused a decay of fluorescence to half-maximal intensity) were determined for four distinct fr...
The activity of LE10 peptide on biological membranes using molecular dynamics, ...
Antunes, Egipto; Azoia, Nuno G.; Matamá, Maria Teresa; Gomes, Andreia; Paulo, Artur CavacoCell-penetrating peptides (CPPs) and antimicrobial peptides (AMPs) are generally defined as small cationic peptides with the ability to interact with lipidic membranes, in a process driven by electrostatic and hydrophobic processes. The interaction with CPPs is known to lead to its translocation across the membrane, while with AMPs lead to membrane damage. Here we present one synthetic anionic peptide, LE10 (L...
In vitro induction of melanin synthesis and extrusion by tamoxifen
Matamá, Maria Teresa; Araújo, Rita; Preto, Ana; Paulo, Artur Cavaco; Gomes, A. C.Physical appearance has significant importance psychologically and socially, with skin and hair being of prime relevance. Effective ingredients that modulate melanin synthesis are of growing interest. Tamoxifen, a widely used selective oestrogen receptor modulator, SERM, was described occasionally in medical case reports as causing grey hair repigmentation. This work aimed to study, in vitro, the effect of tamo...
Chitosan–lignosulfonates sono-chemically prepared nanoparticles : characterisat...
Kim, Suyeon; Fernandes, Margarida M.; Matamá, Maria Teresa; Loureiro, A.; Gomes, Andreia; Paulo, Artur CavacoDue to their recognised properties of biocompatibility, biodegradability and sustainability, chitosan nanocarriers have been successfully used as new delivery systems. In this work, nanoparticles combining chitosan and lignosulfonates were developed for the first time for cosmetic and biomedical applications. The ability of lignosulfonates to act as a counter polyion for stabilisation of chitosan particles, gen...
Direct enzymatic esterification of cotton and Avicel with wild-type and enginee...
Matamá, Maria Teresa; Casal, Margarida; Paulo, Artur CavacoIn this work, the surface of cellulose, either Avicel or cotton fabric, was modified using cutinases without any previous treatment to swell or to solubilise the polymer. Aiming further improvement of cutinase ester synthase activity on cellulose, an engineered cutinase was investigated. Wild-type cutinase from Fusarium solani and its fusion with the carbohydrate-binding module N1 from Cellulomonas fimi were ab...
Potential of human γD-crystallin for hair damage repair : insights into the mec...
Ribeiro, Artur J.; Matamá, Maria Teresa; Cruz, Célia F.; Gomes, A. C.; Paulo, Artur CavacoThe objective of this work was to develop a new strategy to physically ‘repair’ chemically damaged hair. Hence the human eye γD-crystallin, a protein from the superfamily characterized structurally by the Greek key motif, was studied. The human γD-crystallin was chosen based on the ability of proteins belonging to this superfamily to be involved in the coating of specific structures. Two crystallins were used o...
Functionalization of gauzes with liposomes entrapping an anti-inflammatory drug...
Ferreira, Helena; Matamá, Maria Teresa; Silva, Raquel; Silva, Carla Manuela; Gomes, A. C.; Paulo, Artur CavacoFrom ancientness, suitable materials have been developed to cover the wounds in order to prevent infections and promote proper wound healing. In this study, the successful development of functionalized nonwoven gauzes with liposomes entrapping anti-inflammatory piroxicam is reported. Piroxicam is a non-steroidal anti-inflammatory drug (NSAID) that can suppress a persistent inflammatory response, leading to impr...
Lipases efficiently stearate and cutinases acetylate the surface of arabinoxyla...
Stepan, A. M.; Anasontzis, G. E.; Matamá, Maria Teresa; Paulo, Artur Cavaco; Olsson, L.; Gatenholm, P.This is the first report on successful enzyme catalyzed surface esterification of hemicellulose films. Enzyme catalyzed surface acetylation with vinyl acetate and stearation with vinyl stearate were studied on rye arabinoxylan (AX) films. Different surface analytical techniques (FT-IR, TOF-SIMS, ESCA, CA) show that lipases from Mucor javanicus, Rhizopus oryzae and Candida rugosa successfully surface stearate AX...
Production of heterologous cutinases by E. coli and improved enzyme formulation...
Gomes, Daniela S.; Matamá, Maria Teresa; Paulo, Artur Cavaco; Campos-Takaki, Galba M.; Salgueiro, Alexandra A.Background: The hydrolytic action of cutinases has been applied to the degradation of plastics. Polyethylene terephthalate (PET) have long half-life which constitutes a major problem for their treatment as urban solid residues. The aim of this work was to characterize and to improve stable the enzyme to optimize the process of degradation using enzymatic hydrolysis of PET by recombinant cutinases. Results: The ...
Interactions between glycerol, PEG-200 and (NH4)2 SO4 in the stability of heter...
Gomes, Daniela S.; Matamá, Maria Teresa; Paulo, Artur Cavaco; Jordão, R. C. C.; Takaki, G. M. Campos; Salgueiro, Alexandra A.Cutinases (EC 3.1.1.74)are versatile enzymes that have hydrolytic activity on various esters [1]. The spacial structure and the catalytic site of the enzymes can be protected by chemical additives to promove the stability of the activity [2, 3]. The goal of this work was to improve the stability of a recombinant cutinase produced by Escherichia coli.
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