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Application of a high-throughput process analytical technology metabolomics pip...

Abstract Metabolomics aims at gathering the maximum amount of metabolic information for a total interpretation of biological systems. A process analytical technology pipeline, combining gas chromatography�mass spectrometry data preprocessing with multivariate analysis, was applied to a Port wine �forced ageing� process under different oxygen saturation regimes at 60 �C. It was found that extreme �forced ageing�...

Saccharomyces cerevisiae oxidative response evaluation by cyclic voltammetry an...

This study is focused on the evaluation of the impact of Saccharomyces cerevisiae metabolism in the profile of compounds with antioxidant capacity in a synthetic wine during fermentation. A bioanalytical pipeline, which allows for biological systems fingerprinting and sample classification by combining electrochemical features with biochemical background, is proposed. To achieve this objective, alcoholic fermen...

Genotypic and pheno-metabolomic characterization of a Saccharomyces cerevisiae ...

The objective of the present work was to gain a deeper understanding of the phenotypic diversity of natural isolates of Saccharomyces cerevisiae, using high-throughput methods in combination with bioanalytical data. A S. cerevisiae strain collection was constituted comprising 187 strains from different geographical origins and technological uses. Some microsatellite alleles and phenotypes were identified as res...

Characterization of wine fermentations using fiber optic-mediated UV-VIS-SWNIR ...

Spectroscopy is widely used in biological sciences, being applied to liquids, pastes, powders, films, fibers, gases and surfaces. It makes possible to characterize proteins, peptides, lipids, membranes and carbohydrates in pharmaceuticals, foods, plants or animal tissues. It can also provide detailed information about the structure and mechanism of action of molecules. UV-VIS-SWNIR spectroscopy has not been use...

The genotypic and pheno-metabolomic landscape of a Saccharomyces cerevisiae str...

Concepção e desenvolvimento de sistemas biotecnológicos

In a simple and succinct form the authors intend to present the research and development activities that are being undertaken at the University of Minho in the area of biotechnology applied to the study, design, development and construction of equipment goods, which are being carried out together with important Iberian and European industrial companies. The projects are as follows: • Real time monitoring of bee...

Saccharomyces cerevisiae oxidative response by cyclic voltammetry and GC-MS

Moderate electric fields can inactivate Escherichia coli at room temperature

The inactivation of Escherichia coli using moderate electric fields (MEF) below 25 °C, was investigated. Keeping the temperature always below 25 °C demonstrated that electric fields are involved in the inactivation of E. coli, without possible synergistic temperature effects. Electric fields above 220 V cm−1 promoted death rates of 3 log10 cycles of E. coli in less than 6 min, and even higher rates at greater e...

Catalytic ozonation of phenolic acids over a Mn-Ce-O catalyst

Catalytic ozonation of a simulated phenolic wastewater composed by six acids: syringic, vanillic, 3,4,5-trimethoxybenzoic, veratric, protocatechuic and 4-hydroxybenzoic normally present in olive mill wastewaters was studied over a Mn-Ce-O laboratorial catalyst under different operating conditions. A high improvement in total organic carbon (TOC) degradation was observed even when low catalyst concentrations wer...

The X-windows high-throughput mass spectroscopy pipeline