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Expression system of CotA-laccase for directed evolution and high-throughput sc...
Brissos, V.; Pereira, Luciana; Munteanu, Florentina-Daniela; Paulo, Artur Cavaco; Martins, L. O.Laccases are useful biocatalysts for many diverse biotechnological applications. In this study we have established efficient and reliable expression systems and high-throughput screenings for the recombinant CotA-laccase from Bacillus subtilis. The expression levels of cotA-laccase were compared in five different Escherichia coli host strains growing in 96-well microtiter plates under different culture conditio...
Data: 2009
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Origem: RepositóriUM - Universidade do Minho
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