Encontrados 11 documentos, a visualizar página 1 de 2
In vitro degradation and in vivo biocompatibility of chitosan-poly(butylene suc...
Pinto, A. R.; Martins, Ana M.; Carlos, Magda; Correlo, V. M.; Sol, P.; Longatto Filho, Adhemar; Battacharya, Mrinal; Reis, R. L.; Neves, N. M.In tissue engineering, the evaluation of the host response to the biomaterial implantation must be assessed to determine the extent of the inflammatory reaction. We studied the degradation of poly(butylene succinate) and chitosan in vitro using lipase and lysozyme enzymes, respectively. The subcutaneous implantation of the scaffolds was performed to assess tissue response. The type of inflammatory cells present...
Gradual pore formation in natural origin scaffolds throughout subcutaneous impl...
Martins, Ana M.; Kretlow, J. D.; Pinto, A. R.; Malafaya, P. B.; Fernandes, E. M.; Neves, N. M.; Alves, Catarina M.; Mikos, Antonios G.This study used a rat subcutaneous implantation model to investigate gradual in situ pore formation in a self-regulating degradable chitosan-based material, which comprises lysozyme incorporated into biomimetic calcium phosphate (CaP) coatings at the surface to control the scaffold degradation and subsequent pore formation. Specifically, the in vivo degradation of the scaffolds, the in situ pore formation, and ...
Responsive and In situ-forming chitosan scaffolds for bone tissue engineering a...
Martins, Ana M.; Alves, C. M.; Kasper, F. Kurtis; Mikos, Antonios G.; Reis, R. L.The use of bioabsorbable polymeric scaffolds is being investigated for use in bone tissue engineering applications, as their properties can be tailored to allow them to degrade and integrate at optimal rates as bone remodelling is completed. The main goal of this review is to highlight the “intelligent” properties exhibited by chitosan scaffolds and their use in the bone tissue engineering field. To complement ...
Combination of enzymes and flow perfusion conditions improves osteogenic differ...
Martins, Ana M.; Saraf, A.; Sousa, R. A.; Alves, C. M.; Mikos, Antonios G.; Kasper, F. Kurtis; Reis, R. L.Previous studies have shown that a-amylase and lipase are capable of enhancing the degradation of fiber meshes blends of starch and poly(e-caprolactone) (SPCL) under dynamic conditions, and consequently to promote the proliferation and osteogenic differentiation of bone marrow stromal cells (MSCs). This study investigated the effect of flow perfusion bioreactor culture in combination with enzymes on the osteoge...
The role of lipase and alpha-amylase in the degradation of starch/poly(epsilon-...
Martins, Ana M.; Pham, Q. P.; Malafaya, P. B.; Sousa, R. A.; Gomes, Manuela E.; Raphael, R. M.; Kasper, F. Kurtis; Reis, R. L.; Mikos, Antonios G.The present work studies the influence of hydrolytic enzymes (a-amylase or lipase) on the degradation of fiber mesh scaffolds based on a blend of starch and poly(e-caprolactone) (SPCL) and the osteogenic differentiation of osteogenic medium–expanded rat bone marrow stromal cells (MSCs) and subsequent formation of extracellular matrix on these scaffolds under static culture conditions. The biodegradation profile...
Chitosan scaffolds incorporating lysozyme into CaP coatings produced by a biomi...
Martins, Ana M.; Pereira, Rui C.; Leonor, I. B.; Azevedo, Helena S.; Reis, R. L.This study describes an innovative self-regulated degrading material with gradual in situ pore formation ability for bone tissue engineering applications. This approach is based on the incorporation of the lysozyme enzyme into calcium phosphate (CaP) coatings, prepared on the surface of chitosan scaffolds by means of a biomimetic coating technique with the aim of controlling their degradation rate and subsequen...
Natural stimulus responsive scaffolds/cells for bone tissue engineering : influ...
Martins, Ana M.; Pham, Q. P.; Malafaya, P. B.; Raphael, R. M.; Kasper, F. Kurtis; Reis, R. L.; Mikos, Antonios G.This work proposes the use of nonporous, smart, and stimulus responsive chitosan-based scaffolds for bone tissue engineering applications. The overall vision is to use biodegradable scaffolds based on chitosan and starch that present properties that will be regulated by bone regeneration, with the capability of gradual in situ pore formation. Biomimetic calcium phosphate (CaP) coatings were used as a strategy t...
Toward osteogenic differentiation of marrow stromal cells and in vitro producti...
Martins, Ana M.; Alves, C. M.; Reis, R. L.; Mikos, Antonios G.; Kasper, F. KurtisUncorrected proof ; Tissue engineering has emerged as a new interdisciplinary field for the repair of various tissues, restoring their functions by using scaffolds, cells, and/or bioactive factors. A temporary scaffold acts as an extracellular matrix analog to culture cells and guide the development of new tissue. In this chapter, we discuss the preparation of naturally derived scaffolds of polysaccharide orig...
Natural origin scaffolds with in situ pore forming capability for bone tissue e...
Martins, Ana M.; Santos, M. I.; Azevedo, Helena S.; Malafaya, P. B.; Reis, R. L.This work describes the development of a biodegradable matrix, based on chitosan and starch, with the ability to form a porous structure in situ due to the attack by specific enzymes present in the human body (a-amylase and lysozyme). Scaffolds with three different compositions were developed: chitosan (C100) and chitosan/starch (CS80-20, CS60-40). Compressive test results showed that these materials exhibit ve...
Medos normais de crianças em contexto hospitalar segundo o fear survey schedule...
Sampaio, Francisco M.; Martins, Ana M.; Oliveira, Tânia C.Este estudo analisa os medos normais ou desenvolvimentais de 79 crianças (40 meninas e 39 rapazes, idades 6 a 12 anos) frequentadoras de serviços de pediatria (consulta externa e internamento) de dois hospitais portugueses através do Fear Survey Schedule for Children-Revised (fssc-r) de ollendick (1983). Os resultados indicam que as meninas e as crianças com experiência de internamento apresentam mais medos que...
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