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Polymerization of lignosulfonates by the laccase-HBT (1-hydroxybenzotriazole) s...

Prasetyo, E. N.; Kudanga, T.; Østergaard, L.; Rencoret, J.; Gutiérrez, A.; Río, J. C. del; Santos, J. I.; Nieto, L.; Jiménez-Barbero, Jesús

The ability of laccases from Trametes villosa (TvL), Myceliophthora thermophila (MtL), Trametes hirsuta (ThL) and Bacillus subtilis (BsL) to improve the dispersion properties of calcium lignosulfonates 398 in the presence of HBT as a mediator was investigated. Size exclusion chromatography showed an extensive increase in molecular weight of the samples incubated with TvL and ThL by 107% and 572% from 28400 Da a...


Bioelectrochemical investigations of aryl-alcohol oxidase from Pleurotus eryngii

Munteanu, Florentina-Daniela; Ferreira, Patrícia; Ruiz-Duenas, Francisco J.; Martínez, Angel T.; Paulo, Artur Cavaco

Article in Press ; Aryl-alcohol oxidase (AAO) electrochemistry studies, using graphite-modified electrodes, are presented for the first time herein. The increase in current upon injection of the analyzed substrate was shown to be approximated by Michaelis–Menten type dependence. The calculated kinetic constants were used to characterize the native (non-mutated) recombinant AAO expressed in Escherichia coli, as...


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