Encontrados 13 documentos, a visualizar página 1 de 2
Decavanadate, decaniobate, tungstate and molybdate interactions with sarcoplasm...
Fraqueza, Gil; Carvalho, Luís A. E. Batista de; Marques, M. Paula M.; Maia, Luisa; Ohlin, C. André; Casey, William H.; Aureliano, M.Recently we demonstrated that the decavanadate (V10) ion is a stronger Ca2+-ATPase inhibitor than other oxometalates, such as the isoelectronic and isostructural decaniobate ion, and the tungstate and molybdate monomer ions, and that it binds to this protein with a 1 : 1 stoichiometry. The V10 interaction is not affected by any of the protein conformations that occur during the process of calcium translocation ...
Analytical Approaches toward Successful Human Cell Metabolome Studies by NMR Sp...
Duarte, Iola F.; Marques, Joana; Ladeirinha, Ana F.; Rocha, Cláudia; Lamego, Inês; Calheiros, Rita; Silva, Tânia M.; Marques, M. Paula M.The aim of this work was to investigate the effects of cell handling and storage on cell integrity and 1H high resolution magic angle spinning (HRMAS) NMR spectra. Three different cell types have been considered (lung tumoral, amniocytes, and MG-63 osteosarcoma cells) in order for sample-dependent effects to be identified. Cell integrity of fresh cells and cells frozen in cryopreservative solution was 70−80%, w...
Voltammetric and DFT Studies on Viloxazine: Analytical Application to Pharmaceu...
Garrido, E. Manuela P. J.; Garrido, Jorge M. P. J.; Esteves, Mário; Santos-Silva, Alice; Marques, M. Paula M.; Borges, FernandaThe oxidative behavior of viloxazine was studied at a glassy carbon electrode in different buffer systems using cyclic, differential pulse and square-wave voltammetry. The oxidation process was shown to be diffusion-controlled and irreversible over the studied pH. The voltammetric study of the model compounds, 2-ethoxyanisole and morpholine, associated with quantum mechanical (DFT) calculations, allowed to eluc...
Electrochemical and spectroscopic characterisation of amphetamine-like drugs: A...
Milhazes, Nuno; Martins, Pedro; Uriarte, Eugenio; Garrido, Jorge; Calheiros, Rita; Marques, M. Paula M.; Borges, FernandaA complete physicochemical characterisation of MDMA and its synthetic precursors MDA, 3,4-methylenedioxybenzaldehyde (piperonal) and 3,4-methylenedioxy-[beta]-methyl-[beta]-nitrostyrene was carried out through voltammetric assays and Raman spectroscopy combined with theoretical (DFT) calculations. The former provided important analytical redox data, concluding that the oxidative mechanism of the N-demethylation...
Drug-excipient interactions in ketoprofen: A vibrational spectroscopy study
Carvalho, L. A. E. Batista de; Marques, M. Paula M.; Tomkinson, JohnKetoprofen (3-benzoyl-alpha-methylbenzeneacetic acid) is a widely used nonsteroidal anti-inflammatory drug (NSAID), always administered in the form of drug-excipient physical mixtures (PMs). The occurrence of possible interactions between ketoprofen and two commonly used excipients - lactose (LAC) and polyvinylpyrrolidone (PVP) - was evaluated, through vibrational spectroscopy techniques [both Raman and Inelast...
Transverse Acoustic Modes of Biogenic and α,ω-Polyamines: A Study by Inelastic...
Carvalho, Luís A. E. Batista de; Marques, M. Paula M.; Tomkinson, JohnA complete analysis of the transverse acoustic modes (TAMs) for the homologous series of α,ω-diamines (H2N(CH2)nNH2) (n = 2−10, n = 12) as well as for the biogenic polyamines spermidine and spermine was undertaken, by Raman and inelastic neutron scattering (INS) spectroscopies combined with density functional theory (DFT) calculations. A complete assignment of the whole set of TAMs was carried out, for both the...
Spectroscopic and Theoretical Studies on Solid 1,2-Ethylenediamine Dihydrochlor...
Amado, Ana M.; Otero, Juan Carlos; Marques, M. Paula M.; Carvalho, Luís A. E. Batista deA structural study of [H3N(CH2)2NH3)]2+sdot2Cl-, the smallest element of the homologous series of the alpha,omega-diamine dihydrochlorides, was carried out by means of Raman and FTIR spectroscopy coupled to ab initio molecular orbital (MO) calculations. As a primary concern, an adequate molecular model for the representation of these solid amine salts was chosen. Thus, several models, varying in the number and ...
Cytotoxic Activity of Metal Complexes of Biogenic Polyamines: Polynuclear Plat...
Teixeira, Luísa J.; Seabra, Marta; Reis, Elisa; Cruz, M. Teresa Girão da; Lima, M. Conceição Pedroso de; Pereira, Eulália; Miranda, M. AdelaideSeveral polynuclear Pt(II) chelates with biogenic polyamines were synthesized and screened for their potential antiproliferative and cytotoxic activity in different human cancer cell lines. To gather information regarding the structure−activity relationships underlying their biological activity, the complexes studied were designed to differ in geometrical parameters such as the nature of the ligand and the numb...
Study of Biogenic and α,ω-Polyamines by Combined Inelastic Neutron Scattering a...
Marques, M. Paula M.; Carvalho, Luís A. E. Batista de; Tomkinson, JohnA study of the biogenic polyamines spermidine and spermine, as well as of the diamines H2N(CH2)nNH2 (n = 2−10 and n = 12), was carried out by both inelastic neutron scattering (INS) and Raman spectroscopies, for both their undeuterated and N-deuterated forms. Ab initio density functional theory (DFT) methods were also used, to obtain the calculated vibrational spectra of those molecules. A thorough vibrational ...
Effects of Carvedilol on Isolated Heart Mitochondria: Evidence for a Protonopho...
Oliveira, Paulo J.; Marques, M. Paula M.; Carvalho, Luís A. E. Batista de; Moreno, A. J. M.Carvedilol (1-[carbazolyl-(4)-oxy]-3-[2-methoxyphenoxyethyl)amino]-propanol-(2)) is a novel compound used in clinical practice for the treatment of congestive heart failure, mild to moderate hypertension, and myocardial infarction. Carvedilol was also shown to protect cardiac mitochondria from oxidative stress events. Because mitochondria are the main suppliers of ATP for cardiac muscle work, a study of the eff...
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