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Haematology, genotoxicity, enzymatic activity and histopathology as biomarkers ...
Sánchez-Chardi, A.; Marques, C. C.; Gabriel, S. I.; Capela-Silva, F.; Cabrita, A. S.; López-Fuster, M. J.; Nadal, J.; Mathias, M. L.Haematological (WBC, RBC, Hgb and Hct) and genotoxicity (MNT) parameters, hepatic enzymatic activities (GST, GPx and GR), and a histopathological evaluation of liver, kidneys and gonads were assessed as general biomarkers of metal pollution in the shrew Crocidura russula inhabiting a pyrite mining area. Specimens exposed to metals presented a few significant alterations when compared with reference animals: GST...
Besnoitia besnoiti impact on fertility of cattle exploited in mediterranean pas...
Cortes, Helder; Chagas e Silva, J.; Baptista, M.C.; Pereira, R.M.; Leitão, A.; Horta, A.E.M.; Vasques, M.I.; Barber, J.; Marques, C.C.Besnoitia besnoiti is a bovine parasite endemic in many tropical and subtropical areas whose prevalence in the Mediterranean countries such as Portugal seems to be increasing. Most infections are mild or subclinical, characterized by the formation of numerous cutaneous and sub-cutaneous microcysts, lowering the quality of skins for the leather industry. Male sterility or impaired fertility is a common sequela i...
Cyclooxygenase-2 (COX-2) expression by in vitro produced bovine embryos. Prelim...
Marques, C.C.; Horta, A.E.; Vasques, M.I.; Baptista, M.C.; Becker, J.D.; Pimenta, J.; Pereira, R.M.Expression of the two isoforms of cyclooxygenase enzyme, cyclooxygenase-1 (COX-1) and cyclooxygenase-2 (COX-2), by in vitro produced bovine embryos was examined. Cumulus-oocyte complexes were recovered from ovaries of slaughtered animals and subsequently in vitro matured and in vitro fertilized. Presumptive zygotes were cultured in serum containing medium (TCM 199+10% bovine superovulated oestrus serum) on a gr...
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