Two immunoglobulin G enzyme-linked immunosorbent assay (ELISA) versions using whole promastigotes of Leishmania infantum (syn. Leishmania chagasi) treated either with β-mercaptoethanol (β-ME-ELISA) or trypsin (TRYP-ELISA) as antigens were developed for the diagnosis of canine visceral leishmaniasis (CVL). By comparison with the direct agglutination test (DAT; 100%, 31/31; 95% confidence interval [CI]: 86.3–100%...
A Gestão do Risco define-se como a cultura, processos e estruturas que sustentam a gestão efetiva de potenciais oportunidades e efeitos adversos, implicando a definição e a utilização de vários instrumentos que permitem a identificação e a avaliação dos riscos (gestão de risco proactiva) e a deteção e análise de incidentes (gestão de risco reativa). A premência de disponibilizar formação sobre Gestão do Risco e...
As quedas têm um impacto na morbilidade e na qualidade de vida do doente, contribuindo para o aumento dos custos dos cuidados de saúde. Dos múltiplos fatores que podem contribuir para as quedas, destaca-se a idade (acima dos 65 anos), o estado mental, a medicação que o doente está a tomar, história de queda anterior e fatores ambientais. O Centro Hospitalar de Lisboa Central no âmbito da Gestão do Risco monitor...
The anti-HIV drug abacavir is associated with idiosyncratic hypersensitivity reactions and cardiotoxicity. Although the mechanism underlying abacavir-toxicity is not fully understood, drug bioactivation to reactive metabolites may be involved. This work was aimed at identifying abacavir-protein adducts in the hemoglobin of HIV patients as biomarkers of abacavir bioactivation and protein modification. The protoc...
Glomerular filtration rate (GFR) is an accepted measure for assessment of kidney function. For the critically ill patient, creatinine clearance is the method of reference for the estimation of the GFR, although this is often not measured but estimated by equations (i.e., Cockroft-Gault or MDRD) not well suited for the critically ill patient. Functional evaluation of the kidney rests in serum creatinine (Crs) th...
The presence of anti-Ro52 antibodies has been reported in a wide variety of autoimmune diseases, particularly in myositis, scleroderma, and autoimmune liver diseases. Clinical significance of anti-Ro52 antibodies remains controversial, and studies are lacking for clarifying the association of anti-Ro52 with interstitial lung disease (ILD) in connective tissue diseases (CTD). Objectives. To determine if anti-Ro5...
Abstract Introduction: The presence of anti-Ro52 antibodies has been reported in a wide variety of autoimmune diseases, particularly in myositis, scleroderma and autoimmune liver diseases. Clinical significance of anti-Ro52 antibodies remains controversial. Studies are lacking in clarifying the association of anti-Ro52 with pulmonary involvement in scleroderma. Objectives: To determine if anti-Ro52 antibodies a...
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