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Effect of whey protein purity and glycerol content upon physical properties of ...
Ramos, Óscar L.; Reinas, Isabel; Silva, Sara I.; Fernandes, João C.; Cerqueira, M. A.; Pereira, Ricardo; Vicente, A. A.; Poças, M. FátimaThis manuscript describes the detailed characterization of edible films made from two different protein products e whey protein isolate (WPI) and whey protein concentrate (WPC), added with three levels of glycerol (Gly) e i.e. 40, 50 and 60%(w/w). The molecular structure, as well as barrier, tensile, thermal,surface and optical properties of said films were determined, in attempts to provide a better understand...
Evaluation of the Antioxidant Activity of Cell Extracts from Microalgae
Guedes, A. Catarina; Gião, Maria S.; Seabra, Rui; Ferreira, António César Silva; Tamagnini, Paula; Moradas-Ferreira, Pedro; Malcata, F. XavierA growing market for novel antioxidants obtained from non-expensive sources justifies educated screening of microalgae for their potential antioxidant features. Characterization of the antioxidant profile of 18 species of cyanobacteria (prokaryotic microalgae) and 23 species of (eukaryotic) microalgae is accordingly reported in this paper. The total antioxidant capacity, accounted for by both water- and lipid-s...
Features and performance of edible films, obtained from whey protein isolate fo...
Ramos, Óscar L.; Silva, Sara I.; Soares, José C.; Fernandes, João C.; Poças, M. Fátima; Pintado, Manuela E.; Malcata, F. XavierThe goal of this research effort was to assess the efficacy of edible films produced fromwhey protein isolate (WPI) and glycerol, including incorporation of lactic acid (LA) and propionic acid (PRO), chitooligosaccharideswith nominal MW of 3 kDa (COS) and natamycin (NA) as antimicrobial agents. Their features were evaluated in vitro via agar diffusion and viable cell counting, against spoilage microflora often ...
Toxicity of cadmium and zinc on two microalgae, Scenedesmus obliquus and Desmod...
Monteiro, Cristina M.; Fonseca, Susana C.; Castro, Paula M. L.; Malcata, F. XavierAquatic environments often contain toxic heavy metals that may enter the food web via uptake by microalgae and eventually cause severe poisoning problems at higher trophic levels. The effects of Cd and Zn cations upon growth of two native green microalgal species, Scenedesmus obliquus and Desmodesmus pleiomorphus (previously isolated from a polluted site in Northern Portugal), were accordingly evaluated. Growth...
The potential effect of FOS and inulin upon probiotic bacterium performance in ...
Rodrigues, Dina; Rocha-Santos, Teresa A. P.; Pereira, Cláudia I.; Gomes, Ana M.; Malcata, F. Xavier; Freitas, Ana C.Inulin and fructooligosaccharides were studied for their prebiotic effect upon growth/survival of probiotic bacteria and technological potential in probiotic food processing, via characterization of glycolysis, proteolysis and lipolysis in curdled milk matrices; the ultimate goal is the manufacture of synbiotic cheeses. Prebiotic compounds did not significantly affect growth/viability of all strains studied, ex...
Influence of L-cysteine, oxygen and relative humidity upon survival throughout ...
Rodrigues, D.; Sousa, S.; Rocha-Santos, T.; Silva, J.P.; Lobo, J.M. Sousa; Costa, P.; Amaral, M. H.; Pintado, M. M.; Gomes, A. M.; Malcata, F. X.The survival rates of Lactobacilus acidophilus Ki, Lactobacillus paracasei L26 and Bifidobacterium animalis BB-12 were studied after whey protein microencapsulation via spray-drying, with or without L-cysteine- HCl, and storage up to 6 months at 5 C and 22 C, with variation in relative air humidity and oxygen levels. Lb. paracasei L26 was the least susceptible to storage conditions: above 106 cfu g 1 were rec...
Quantitative and qualitative determination of CLA produced by bifidobacterium a...
Rodríguez-Alcalá, Luis M.; Braga, Teresa; Malcata, F. Xavier; Gomes, Ana; Fontecha, JavierBifidobacterium and lactic acid bacteria (LAB), especially from the genera Lactobacillus and Lactococcus, are commonly used in the production of fermented dairy products due to their potential probiotic characteristics. Moreover, some strains of these microorganisms also have the ability to produce conjugated linoleic acid (CLA) from linoleic acid (LA), which has attracted much attention as a novel type of bene...
Novel whey-derived peptides with inhibitory effect against angiotensin-converti...
Tavares, Tânia; Del Mar Contreras, Maria; Amorim, Manuela; Pintado, Manuela; Recio, Esidra; Malcata, F. XavierWhey protein concentrate (WPC) was subjected to enzymatic hydrolysis by proteases from the flowers of Cynara cardunculus, and the resulting angiotensin-converting enzyme (ACE)-inhibitory effect was monitored. The whole WPC hydrolysate exhibited an IC50 value of 52.9±2.9ºg/mL, whereas the associated peptide fraction with molecular weight below 3 kDa scored 23.6±1.1ºg/mL. The latter fraction was submitted to RP-H...
Optimisation, by response surface methodology, of degree of hydrolysis and anti...
Tavares, T. G.; Contreras, M. M.; Amorim, M.; Martín-Álvarez, P. J.; Pintado, M. E.; Recio, I.; Malcata, F. X.The hydrolysis of bovine whey protein concentrate (WPC), a-lactalbumin (a-La) and caseinomacropeptide (CMP), by aqueous extracts of Cynara cardunculus, was optimized using response surface methodology. Degree of hydrolysis (DH), angiotensin-converting enzyme (ACE)-inhibitory activity and antioxidant activity were used as objective functions, and hydrolysis time and enzyme/substrate ratio as manipulated paramete...
Antiulcerogenic activity of peptide concentrates obtained from hydrolysis of wh...
Tavares, T.G.; Monteiro, K.M.; Possenti, A.; Pintado, M.E.; Carvalho, J.E.; Malcata, F.X.Peptide concentrates generated by hydrolysis of whey with aqueous extracts of flowers of Cynara cardunculus were studied for possible protection of the stomach mucosa against ulcerative lesions caused by oral administration of absolute ethanol. Both the whole peptide fraction obtained via hydrolysis of whey protein concentrate (peptide concentrate, PepC) and its fraction below 3 kDa (PepCF) were able to reduce ...
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