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Effect of whey protein purity and glycerol content upon physical properties of ...
Ramos, Óscar L.; Reinas, Isabel; Silva, Sara I.; Fernandes, João C.; Cerqueira, M. A.; Pereira, Ricardo; Vicente, A. A.; Poças, M. FátimaThis manuscript describes the detailed characterization of edible films made from two different protein products e whey protein isolate (WPI) and whey protein concentrate (WPC), added with three levels of glycerol (Gly) e i.e. 40, 50 and 60%(w/w). The molecular structure, as well as barrier, tensile, thermal,surface and optical properties of said films were determined, in attempts to provide a better understand...
Evaluation of the Antioxidant Activity of Cell Extracts from Microalgae
Guedes, A. Catarina; Gião, Maria S.; Seabra, Rui; Ferreira, António César Silva; Tamagnini, Paula; Moradas-Ferreira, Pedro; Malcata, F. XavierA growing market for novel antioxidants obtained from non-expensive sources justifies educated screening of microalgae for their potential antioxidant features. Characterization of the antioxidant profile of 18 species of cyanobacteria (prokaryotic microalgae) and 23 species of (eukaryotic) microalgae is accordingly reported in this paper. The total antioxidant capacity, accounted for by both water- and lipid-s...
Features and performance of edible films, obtained from whey protein isolate fo...
Ramos, Óscar L.; Silva, Sara I.; Soares, José C.; Fernandes, João C.; Poças, M. Fátima; Pintado, Manuela E.; Malcata, F. XavierThe goal of this research effort was to assess the efficacy of edible films produced fromwhey protein isolate (WPI) and glycerol, including incorporation of lactic acid (LA) and propionic acid (PRO), chitooligosaccharideswith nominal MW of 3 kDa (COS) and natamycin (NA) as antimicrobial agents. Their features were evaluated in vitro via agar diffusion and viable cell counting, against spoilage microflora often ...
Toxicity of cadmium and zinc on two microalgae, Scenedesmus obliquus and Desmod...
Monteiro, Cristina M.; Fonseca, Susana C.; Castro, Paula M. L.; Malcata, F. XavierAquatic environments often contain toxic heavy metals that may enter the food web via uptake by microalgae and eventually cause severe poisoning problems at higher trophic levels. The effects of Cd and Zn cations upon growth of two native green microalgal species, Scenedesmus obliquus and Desmodesmus pleiomorphus (previously isolated from a polluted site in Northern Portugal), were accordingly evaluated. Growth...
The potential effect of FOS and inulin upon probiotic bacterium performance in ...
Rodrigues, Dina; Rocha-Santos, Teresa A. P.; Pereira, Cláudia I.; Gomes, Ana M.; Malcata, F. Xavier; Freitas, Ana C.Inulin and fructooligosaccharides were studied for their prebiotic effect upon growth/survival of probiotic bacteria and technological potential in probiotic food processing, via characterization of glycolysis, proteolysis and lipolysis in curdled milk matrices; the ultimate goal is the manufacture of synbiotic cheeses. Prebiotic compounds did not significantly affect growth/viability of all strains studied, ex...
Quantitative and qualitative determination of CLA produced by bifidobacterium a...
Rodríguez-Alcalá, Luis M.; Braga, Teresa; Malcata, F. Xavier; Gomes, Ana; Fontecha, JavierBifidobacterium and lactic acid bacteria (LAB), especially from the genera Lactobacillus and Lactococcus, are commonly used in the production of fermented dairy products due to their potential probiotic characteristics. Moreover, some strains of these microorganisms also have the ability to produce conjugated linoleic acid (CLA) from linoleic acid (LA), which has attracted much attention as a novel type of bene...
Novel whey-derived peptides with inhibitory effect against angiotensin-converti...
Tavares, Tânia; Del Mar Contreras, Maria; Amorim, Manuela; Pintado, Manuela; Recio, Esidra; Malcata, F. XavierWhey protein concentrate (WPC) was subjected to enzymatic hydrolysis by proteases from the flowers of Cynara cardunculus, and the resulting angiotensin-converting enzyme (ACE)-inhibitory effect was monitored. The whole WPC hydrolysate exhibited an IC50 value of 52.9±2.9ºg/mL, whereas the associated peptide fraction with molecular weight below 3 kDa scored 23.6±1.1ºg/mL. The latter fraction was submitted to RP-H...
Capacity of simultaneous removal of zinc and cadmium from contaminated media, b...
Monteiro, Cristina M.; Castro, Paula M. L.; Malcata, F. XavierSeveral aquatic environments have been contaminated with heavy metals dumped via industrial effluents. Numerous studies have been published regarding the removal of single metals from aqueous solutions by microalgal biomass. However, such studies do not reflect the actual problem associated with industrial effluents because usually more than one metal species is present. Here we studied the biosorption capacity...
Biosorption of zinc ions from aqueous solution by the microalga Scenedesmus obl...
Monteiro, Cristina M.; Castro, Paula M. L.; Malcata, F. XavierAquatic environments are often exposed to toxic heavy metals, which gain access to the food chain via microalgae and may cause severe problems at higher trophic levels. However, such a metabolic specificity can be taken advantage of in bioremediation strategies. The potential of a novel wild strain of Scenedesmus obliquus, previously isolated from a heavy metal-contaminated site in northern Portugal, to remove ...
Rheological, textural and microstructural features of probiotic whey cheeses
Madureira, A. Raquel; Pintado, Ana I.; Gomes, Ana Maria; Pintado, Manuela E.; Malcata, F. XavierWhey cheeses have been manufactured with probiotic bacteria e viz. Bifidobacterium animalis Bo and Lactobacillus casei LAFTIrL26, from combinations of bovine whey and milk, following protein denaturation at 90 ºC; they were subsequently inoculated (at 10%) with those strains, and homogenized afterwards; additives such as salt and sugar were then incorporated; and the resulting solid matrices were stored at 7º C...
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