Encontrados 25 documentos, a visualizar página 1 de 3
Gradual pore formation in natural origin scaffolds throughout subcutaneous impl...
Martins, Ana M.; Kretlow, J. D.; Pinto, A. R.; Malafaya, P. B.; Fernandes, E. M.; Neves, N. M.; Alves, Catarina M.; Mikos, Antonios G.This study used a rat subcutaneous implantation model to investigate gradual in situ pore formation in a self-regulating degradable chitosan-based material, which comprises lysozyme incorporated into biomimetic calcium phosphate (CaP) coatings at the surface to control the scaffold degradation and subsequent pore formation. Specifically, the in vivo degradation of the scaffolds, the in situ pore formation, and ...
In vivo study of dendron-like nanoparticles for stem cells tune-up : from nano ...
Oliveira, Joaquim M.; Sousa, R. A.; Malafaya, P. B.; Silva, S. S.; Kotobuki, Noriko; Hirose, Motohiro; Ohgushi, Hajime; Mano, J. F.; Reis, R. L.The control of stem cell differentiation to obtain osteoblasts in vivo is still regarded as a challenge in stem-cell-based and bone-tissue engineering strategies. Biodegradable dexamethasone-loaded dendron-like nanoparticles (NPs) of carboxymethylchitosan/poly(amidoamine) dendrimer have been proposed as intracellular drug-delivery systems of bioactive molecules. In this study, combination of nanotechnology, ste...
The effect of insulin-loaded chitosan particle-aggregated scaffolds in chondrog...
Malafaya, P. B.; Oliveira, João T.; Reis, R. L.Osteochondral defect repair requires a tissue engineering approach that aims at mimicking the physiological properties and structure of two different tissues (cartilage and bone) using a scaffold–cell construct. One ideal approach would be to engineer in vitro a hybrid material using a single-cell source. For that purpose, the scaffold should be able to provide the adequate biochemical cues to promote the selec...
Gellan gum : a new biomaterial for cartilage tissue engineering applications
Oliveira, João T.; Martins, L.; Picciochi, R.; Malafaya, P. B.; Sousa, R. A.; Neves, N. M.; Mano, J. F.; Reis, R. L.Gellan gum is a polysaccharide manufactured by microbial fermentation of the Sphingomonas paucimobilis microorganism, being commonly used in the food and pharmaceutical industry. It can be dissolved in water, and when heated and mixed with mono or divalent cations, forms a gel upon lowering the temperature under mild conditions. In this work, gellan gum hydrogels were analyzed as cells supports in the context o...
The role of lipase and alpha-amylase in the degradation of starch/poly(epsilon-...
Martins, Ana M.; Pham, Q. P.; Malafaya, P. B.; Sousa, R. A.; Gomes, Manuela E.; Raphael, R. M.; Kasper, F. Kurtis; Reis, R. L.; Mikos, Antonios G.The present work studies the influence of hydrolytic enzymes (a-amylase or lipase) on the degradation of fiber mesh scaffolds based on a blend of starch and poly(e-caprolactone) (SPCL) and the osteogenic differentiation of osteogenic medium–expanded rat bone marrow stromal cells (MSCs) and subsequent formation of extracellular matrix on these scaffolds under static culture conditions. The biodegradation profile...
Natural stimulus responsive scaffolds/cells for bone tissue engineering : influ...
Martins, Ana M.; Pham, Q. P.; Malafaya, P. B.; Raphael, R. M.; Kasper, F. Kurtis; Reis, R. L.; Mikos, Antonios G.This work proposes the use of nonporous, smart, and stimulus responsive chitosan-based scaffolds for bone tissue engineering applications. The overall vision is to use biodegradable scaffolds based on chitosan and starch that present properties that will be regulated by bone regeneration, with the capability of gradual in situ pore formation. Biomimetic calcium phosphate (CaP) coatings were used as a strategy t...
Bilayered chitosan-based scaffolds for osteochondral tissue engineering : influ...
Malafaya, P. B.; Reis, R. L.Osteochondral tissue engineering presents a current research challenge due to the necessity of combining both bone and cartilage tissue engineering principles. In the present study, bilayered chitosan-based scaffolds are developed based on the optimization of both polymeric and composite scaffolds. A particle aggregation methodology is proposed in order to achieve an improved integrative bone–cartilage interfac...
Novel hydroxyapatite/carboxymethylchitosan composite scaffolds prepared through...
Oliveira, Joaquim M.; Costa, S. A.; Leonor, I. B.; Malafaya, P. B.; Mano, J. F.; Reis, R. L.A developmental composite scaffold for bone tissue engineering applications composed of hydroxyapatite (HA) and carboxymethylchitosan (CMC) was obtained using a coprecipitation method, which is based on the ‘‘autocatalytic’’ electroless deposition route. The results revealed that the pores of the scaffold were regular, interconnected, and possess a size in the range of 20–500 lm. Furthermore, the Fourier transf...
Macroporous hydroxyapatite scaffolds for bone tissue engineering applications :...
Oliveira, Joaquim M.; Silva, S. S.; Malafaya, P. B.; Rodrigues, Márcia T.; Kotobuki, Noriko; Hirose, Motohiro; Gomes, Manuela E.; Mano, J. F.In this work, a new methodology is reported for developing hydroxyapatite (HA) scaffolds using an organic sacrifice template. The novelty of work consists of possibility of obtaining porous and highly interconnected scaffolds mimicking the sacrificial component. Our purpose consisted of evaluating the physicochemical properties of the HA scaffolds by means of Fourier transform infra-red spectroscopy, X-ray diff...
Osteochondral tissue engineering constructs combining particle aggregated scaff...
Malafaya, P. B.Tese de Doutoramento em Engenharia Biomédica ; Osteochondral defects are lesions of the articular cartilage where the underlying bone tissue is also damaged. Although some studies have achieved success in repairing small cartilage defects, there is no widely accepted method for complete repair of large osteochondral defects. This is due to different factors, for instances, the ones associated with mechanical i...
| Financiadores do RCAAP | |||||||
|
|
|
|
|
|
||