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Protein partitioning in polymer/polymer aqueous two-phase systems

Tese de doutoramento. Engenharia Química e Biológica. Faculdade de Engenharia. Universidade do Porto. 2008

O culpa é dos narradores: os contos fantásticos de Edgar Allan Poe

Tese de mestrado, Teoria da Literatura, Universidade de Lisboa, Faculdade de Letras, 2012 ; Esta tese consiste na análise de alguns contos de Edgar Allan Poe. Nela tento mostrar que os acontecimentos sobrenaturais estão subordinados aos interesses particulares dos narradores culpados de Poe e que, por essa razão, não merecem que o leitor lhes dê crédito. O centro do argumento é uma leitura literal de “Ligeia”....

Building information modeling: oportunidades e desafios para projectistas e don...

Dissertação apresentada na Faculdade de Ciências e Tecnologia da Universidade Nova de Lisboa para obtenção do grau de Mestre em Engenharia Civil-Perfil de Construção ; O sector da arquitectura, engenharia e construção (AEC) é frequentemente apontado como pouco eficiente e produtivo, uma vez que é considerado um sector fragmentado, no qual existe uma enorme relutância em adoptar novas tecnologias que permitam t...

Correlations between distribution coefficients of various biomolecules in diffe...

Distribution coefficients for a variety of proteins and certain other biomolecules (peptides, amino acids, and carbohydrates) (overall 27 different solutes) were measured in aqueous two-phase systems (ATPSs) dextran (Dex)–polyethylene glycol (PEG) and Dex–Ucon 50-HB-5100 (Ucon—a random copolymer of ethylene glycol and propylene glycol) both containing 0.15MNaCl in 0.01Mphosphate buffer, pH 7.4, at 23 ◦C. Distri...

ΔG(CH2) as solvent descriptor in polymer/polymer aqueous two-phase systems

Phase diagrams were determined for aqueous two-phase systems (ATPSs) formed by different paired combinations of Dextran (Dex-75), Ficoll-70, polyethylene glycol (PEG-8000), hydroxypropyl starch (PES-100), and Ucon50HB5100 (a random copolymer of ethylene glycol and propylene glycol) all containing 0.15 M NaCl in 0.01 M phosphate buffer, pH 7.4, at 23 °C. Partition coefficients of a series of dinitrophenylated (D...

Relative hydrophobicity of (PEG or Ucon)-salt ATPSs

Aqueous Two-Phase Systems (ATPSs) are biphasic systems composed mainly by water. ATPSs are obtained upon mixing of two aqueous solutions of certain polymers or a polymer and a salt (above certain critical conditions, e.g. concentration, temperature). These systems are commonly indicated for the extraction of biomolecules. In this work, the partition coefficients for a series of five dinitrophenylated amino-acid...

ΔG(CH2) in PEG-Salt and Ucon-Salt Aqueous Two-Phase Systems

Three new phase diagrams for polymer−salt aqueous two-phase systems (ATPS) at 23 °C are presented. Then, partition coefficients for a series of five dinitrophenylated amino acids were obtained experimentally in five polymer−salt ATPS. The ATPS were obtained combining a polymer, polyethylene glycol (PEG) or Ucon (a random copolymer of 50 % ethylene oxide and 50 % propylene oxide), and a salt (Na2SO4, (NH4)2SO4, ...

“On the collander equation”: protein partitioning in polymer/polymer aqueous tw...

Distribution coefficients of randomly selected proteins were measured in aqueous two-phase systems (ATPSs) formed by different combinations of Dextran-75 (Dex), Ficoll-70, polyethylene glycol-8000 (PEG), hydroxypropyl starch-100 (PES), and Ucon50HB5100 (Ucon, a random copolymer of ethylene glycol and propylene glycol) at particular polymer concentrations, all containing 0.15 M NaCl in 0.01 M phosphate buffer, p...

Physicochemical characterization of the PEG8000-Na2SO4 aqueous two-phase system

The polyethylene glycol-sodium sulfate aqueous two-phase system has been characterized at 23 °C. Tielines for the phase diagram were obtained experimentally. Phases in equilibrium were characterized by means of the solvatochromic parameters π*, α, and β, which provide a measurement of the polarity/polarizability and the H-bond donor and acceptor abilities, respectively. The ability of the phases to participate ...

Relationships between partition coefficients of biomolecules in several aqueous...