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Electrospun silk-elastin-like fiber mats for tissue engineering applications

Protein-based polymers are present in a wide variety of organisms fulfilling structural and mechanical roles. Advances in protein engineering and recombinant DNA technology allow the design and production of recombinant protein-based polymers (rPBPs) with an absolute control of its composition. Although the application of recombinant proteins as biomaterials is still an emerging technology, the possibilities ar...

A synthetic biology approach for the development of recombinant protein-based p...

The search for advanced materials, especially for those bioinspired, has recently been the focus of great research in many fields of application. Due to recent advances in materials characterization and fabrication technologies, and especially through the use of synthetic biology approaches, it is now possible to reengineer novel functionalities and structures of protein-based materials, taking advantage ...

Antibacterial and antifungal activity of a new solvent-cast film based on a fun...

Key stress factors and parameters for batch production optimisation of silk-ela...

Solvent-cast films of an elastin-like polymer fused to an antimicrobial peptide...

The nosocomial infections grew significantly in the last years and became a worldwide problem. Antimicrobial peptides (AMPs) arise as a good treatment to these infections, since traditional antibiotics have become useless against resistant hospital strains. AMPs exhibit a broad range of antimicrobial activity but antitumoral and antiviral activities have also been found. AMPs are usually small, cationic molecul...

A colecção de estirpes autóctones de Saccharomyces cerevisiae das principais re...

Livro de Actas on-line em http://www.ateva.pt/8-simposio-de-vitivinicultura-do-alentejo/ ; A levedura Saccharomyces cerevisiae é caracterizada por uma elevada variabilidade fenotípica, que está associada à ocorrência de estirpes em habitats naturais diversificados. Em trabalhos anteriores, demonstrámos a elevada diversidade de estirpes isoladas a partir de ambientes vitivinícolas na Região dos Vinhos Verdes, b...

A colecção de estirpes autóctones de Saccharomyces cerevisiae das principais re...

Apresentação efectuada no "Simpósio de Vitivinicultura do Alentejo, 7, Évora, 2010" ; A levedura Saccharomyces cerevisiae é caracterizada por uma elevada variabilidade fenotípica, que está associada à ocorrência de estirpes em habitats naturais diversificados. Em trabalhos anteriores, demonstrámos a elevada diversidade de estirpes isoladas a partir de ambientes vitivinícolas na Região dos Vinhos Verdes, bem co...

Creating new functional biomaterials : construction and production of Bone Morp...

Bone morphogenetic protein 2 (BMP-2) is a potent osteoinductive cytokine from the TGF-β superfamily that triggers the development of stem cells into osteoblasts. Its therapeutic interest has led to the development of various production systems for recombinant variables of BMP-2. Production has been achieved in expression systems ranging from animal cells to bacteria, but is always associated with three major dr...

Use of elastin like polymer (VPAVG)220 fusion TAGS for recombinant protein, pro...

One of the major challenges to the economical preparation of biomedical and biotechnological recombinant proteins today resides in the availability of an efficient production and purification protocol. Some proteins may not lend themselves to effective purification or may require expensive and cumbersome multistep chromatographic protocols. On the other hand, use of synthetically produced protein based polymer ...

Production and purification of recombinant antimicrobial peptides by exploiting...

The extensive and incorrect use of chemical antibiotics led to a resistance development by pathogens. The antimicrobial peptides (AMPs) arise as a good alternative to traditional pharmaceutical agents. They exhibit a broad range of antimicrobial activity but antitumoral and antiviral activities have also been found. AMPs are usually small, cationic molecules that occur as part of the innate defense mechanism in...