Encontrados 11 documentos, a visualizar página 1 de 2

Optimization of a microplate-based assay to assess esterase activity in the alg...

The present work describes the optimization of a short-term assay, based on the inhibition of the esterase activity of the alga Pseudokirchneriella subcapitata, in a microplate format. The optimization of the staining procedure showed that the incubation of the algal cells with 20 μmolL−1 fluorescein diacetate (FDA) for 40 min allowed discrimination between metabolic active and inactive cells. The shortterm ass...

Evaluation of the role of glutathione in the lead-induced toxicity in saccharom...

The effect of intracellular reduced glutathione (GSH) in the lead stress response of Saccharomyces cerevisiae was investigated. Yeast cells exposed to Pb, for 3 h, lost the cell proliferation capacity (viability) and decreased intracellular GSH level. The Pb-induced loss of cell viability was compared among yeast cells deficient in GSH1 (∆gsh1) or GSH2 (∆gsh2) genes and wild-type (WT) cells. When exposed to Pb,...

Optimization of a microplate-based assay to assess esterase activity in the alg...

The present work describes the optimization of a short-term assay, based on the inhibition of the esterase activity of the alga Pseudokirchneriella subcapitata, in a microplate format. The optimization of the staining procedure showed that the incubation of the algal cells with 20 μmol L−1 fluorescein diacetate (FDA) for 40 min allowed discrimination between metabolic active and inactive cells. The short-term a...

Evaluation of the role of glutathione in the lead-induced toxicity in Saccharom...

The effect of intracellular reduced glutathione (GSH) in the lead stress response of Saccharomyces cerevisiae was investigated. Yeast cells exposed to Pb, for 3 h, lost the cell proliferation capacity (viability) and decreased intracellular GSH level. The Pb-induced loss of cell viability was compared among yeast cells deficient in GSH1 (∆gsh1) or GSH2 (∆gsh2) genes and wild-type (WT) cells. When exposed to Pb,...

Removal of heavy metals using a brewer's yeast strain of Saccharomyces cerevisi...

In the present work, the influence of the competitive effect of inorganic ligands (carbonates, chlorides, fluorides, phosphates, nitrates and sulphates), which can be present in real multi-metal electroplating effluents, on the biosorption of chromium, copper, nickel and zinc ions by yeast cells of Saccharomyces cerevisiae was rationally examined. Additionally, chemical speciation studies allowed optimizing the...

Using a flocculent brewer’s yeast strain of Saccharomyces cerevisiae in the rem...

Biorremediation of heavy metals by a brewer’s yeast

In this work, the suitability of live and inactivated cells of a brewing yeast strain of Saccharomyces cerevisiae NCYC1364 in the removal of Cu2+, Ni2+ and Zn2+ from solution was compared. Kinetics studies showed that 30 minutes of the contact of live or dead cells with all the metals were enough to occur the maximum removal, except for live cells with Cu2+, where the equilibrium was attained after 60 minutes. ...

Bioremediation of nickel from electroplating wastewaters

Treatment of electroplating wastewaters using flocculent cells of Saccharomyces...

Biosorption of heavy metals using flocculent cells of Saccharomyces cerevisiae