Encontrados 5 documentos, a visualizar página 1 de 1
Drugs of abuse induce apoptotic features in PC12 cells.
Oliveira, M Teresa; Rego, A Cristina; Macedo, Tice R; Oliveira, Catarina RDrugs of abuse induce the release of dopamine in the central nervous system, particularly in the mesolimbic-mesocortical pathway. As dopamine may act as a neurotoxin, in this study, we analyzed the effects of the drugs of abuse, cocaine, heroin, and amphetamine, on the neurodegeneration of PC12 cells, a dopaminergic cell line, by evaluating the activity of caspase-3 and mitochondrial cytochrome c release. All t...
Toxic Effects of Opioid and Stimulant Drugs on Undifferentiated PC12 Cells
Oliveira, M Teresa; Rego, A Cristina; Morgadinho, M Teresa; Macedo, Tice R; Oliveira, Catarina RCell death and reactive oxygen species production have been suggested to be involved in neurodegeneration induced by the drugs of abuse. In this study we analyze the toxicity of the following drugs of abuse: heroin, morphine, d-amphetamine, and cocaine in undifferentiated PC12 cells, used as dopaminergic neuronal models. Our data show that opioid drugs (heroin and morphine) are more toxic than stimulant drugs (...
Efeito crónico da heroína e do peróxido de hidrogénio em células PC12
Cunha-Oliveira, Teresa; Rego, A Cristina; Morgadinho, M Teresa; Macedo, Tice R; Oliveira, Catarina RO consumo repetido de drogas, nomeadamente opióides, conduz ao desenvolvimento de dependência. Entre os mecanismos envolvidos neste processo, comuns à maioria das drogas de abuso, destaca-se a activação da neurotransmissão dopaminérgica em zonas específicas do cérebro. O metabolismo oxidativo da dopamina confere-lhe um potencial neurotóxico devido à formação de espécies reactivas de oxigénio, nomeadamente peróx...
Influence of the sample preparation method on the serotonin determination in pl...
Morgadinho, Maria T.; Ribeiro, C. A. Fontes; Macedo, Tice R. A.Plasma or platelet serotonin concentration is commonly used to provide information about the serotonergic activity in various psychiatric or neurological diseases. Some difficulties have been described in the measurement of serotonin (5-HT) levels in plasma or platelets. We describe an isocratic liquid-chromatographic assay with amperometric detection for determination of 5-HT in the platelet pellet and in plat...
New insights into the oxidation pathways of apomorphine
Garrido, Jorge; Delerue-Matos, Cristina; Borges, Fernanda; Macedo, Tice R. A.; Oliveira-Brett, A. M.A detailed study of the oxidative behaviour of apomorphine in aqueous media is reported. Resorting to the synthesis of apomorphine derivatives it was possible to identify all the anodic oxidation peaks of apomorphine, which are related to the oxidation of the catechol and tertiary amine groups. These findings were revealed to be important since they could lead to a better understanding of the biological interac...
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