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Candida albicans virulence and drug-resistance requires the O-acyltransferase G...

Background GUP1 gene was primarily identified in Saccharomyces cerevisiae being connected with glycerol uptake defects in association with osmotic stress response. Soon after, Gup1p was implicated in a complex and extensive series of phenotypes involving major cellular processes. These include membrane and wall maintenance, lipid composition, bud-site selection, cytoskeleton orientation, vacuole morphology, sec...

Yeasts, the man’s best friend

1. Introduction In most cultures, bread making depends on a fermentation step. The flour leavening ability was, at first, most probably dependent on spontaneous fermentation. It became a controlled process by the maintenance of fresh innocula from one preparation to the next and this kind of environmental constraints eventually generated a particular type of yeast and bacteria biodiversity, adapted to ferment ...

Molecular characterization of Saccharomyces cerevisiae extracellular matrix and...

Yeast, Saccharomyces cerevisiae, presents itself as the best studied eukaryotic model, with the possibility to modify its genetic information with highly advanced molecular techniques. This microorganism displays a high degree of similarity and conservation of processes with higher eukaryotes. Several fundamental mechanisms such as cell cycle regulation, DNA replication, recombination and repair were first unco...

The yeast Saccharomyces cerevisiae is sensitive to colorectal cancer routine tr...

Cetuximab/Erbitux® (Merk Sereno), a drug used in routine treatment of colorectal cancer and other malignant pathologies, is a monoclonal antibody against the Epidermal Growth Factor Receptor (EGFR). A frequent problem affecting the clinical use of Cetuximab is the lack of effectiveness deriving from frequent mutations in K-ras (1-3). A set of mutations in K-ras gene, KRAS c.35G>A (G12D), KRAS c.38G>A (G13D) in ...

Programmed cell death processes in Saccharomyces cerevisiae are altered by GUP1...

During the past years, yeast has been successfully established as a model to study mechanisms of programmed cell death regulation. Saccharomyces cerevisiae commits to cell death showing typical hallmarks of metazoan apoptosis, in response to different stimuli. Gup1p, an O-acyltransferase, is required for several cellular processes that are related to apoptosis development, such as rafts integrity and stability,...

Saccharomyces cerevisiae glycerol/H+ symporter Stl1p is essential for cold/near...

Background: Freezing is an increasingly important means of preservation and storage of microbial strains used for many types of industrial applications including food processing. However, the yeast mechanisms of tolerance and sensitivity to freeze or near-freeze stress are still poorly understood. More knowledge on this regard would improve their biotechnological potential. Glycerol, in particular intracellular...

Biologia Molecular, bloco 1 : expressão genética : guião das aulas práticas

Expression studies of GUP1 and GUP2, genes involved in glycerol active transpor...

Glycerol active uptake in Saccharomyces cerevisiae, characterised physiologically as a H+/symport, was previously described as repressed by glucose, induced by growth on non-fermentable carbon sources and unresponsive to growth under salt stress. GUP1 and GUP2 were identified and characterised as genes involved in glycerol active uptake. Using SQ-RT-PCR, GUP1 and GUP2 transcription was measured. Unlike active t...

Debaryomyces hansenii Xylhp gene, partial cds

Submissão da sequência Dh.XYLH à base de dados do GenBank do NCBI.

Yeast orthologues associated with glycerol transport and metabolism

Glycerol is a key compound in the regulation of several metabolic pathways in Saccharomyces cerevisiae. Most of the genes from this yeast involved in glycerol consumption, production and transport are now available. Some of the mechanisms involving glycerol metabolism and transport are common to other yeasts. This work presents a search for GPD1/2, GUT1, GUP1/2 and FPS1 orthologues. All the genes hereby cloned ...