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Inquéritos de satisfação: Triagem de Manchester no Hospital de Santo António

Molecular aspects and comparative genomics of bacteriophage endolysins

Phages are recognized as the most abundant and diverse entities on the planet. Their diversity is predominantly determined by their dynamic adaptation capacities, when confronted with different selective pressures in an endless cycle of co-evolution with a widespread group of bacterial hosts. At the end of the infection cycle, progeny virions are confronted with a rigid cell wall that hinders their release into...

Controlled RNA contamination and degradation and its impact on qPCR gene expres...

RNA quality is of utmost importance to perform gene expression quantification by qPCR. The classical methods used to determine \RNA\ quality are based on electrophoresis and spectrophotometer assessment, namely A260/A280 and A260/A230 ratios. It was previously shown that due to the complex nature of Staphylococcus epidermidis biofilms, \RNA\ extraction procedures could impact mRNA quality and thus accurate quan...

Complete genome sequence of the broad-host-range Paenibacillus larvae phage phi...

Paenibacillus larvae is a Gram-positive bacterium that causes American foulbrood, an important disease in apiculture. We report the first complete genome sequence of a P. larvae phage, phiIBB_Pl23, isolated from a hive in northern Portugal. This phage belongs to the family Siphoviridae.

Relationship between biofilm formation and antibiotic resistance in commensal i...

Staphylococcus epidermidis is a common bacterial coloniser of the normal human microflora and usually have a benign relationship with the host. For several years, S. epidermidis was regarded as a harmless commensal microorganism. However, this bacterium is now recognised as an opportunistic pathogen, representing a leading cause of healthcare-associated infections. The major recognised determinants in the patho...

Genomic characterization of the Staphylococcus epidermidis-specific bacteriopha...

Staphylococcus epidermidis is one of the most frequent causative agents of nosocomial infections, predominantly in patients with indwelling medical devices. This microrganism may form biofilms which are microbial structures very tolerant to the host immune defenses and to antibiotherapy. Therefore, studies are needed in order to develop effective methods for biofilm control. Currently, bacteriophages (phages) a...

Molecular and functional aspects of bacteriophage endolysins

3D motion control with axis interpolation

Há alguns anos que a automação e controlo entraram no mundo do entretenimento. A evidenciar este facto, tem-se assistido nos últimos anos a um aumento de soluções e inovações nesta área. Tal desafio resulta em grande parte, da exigência dos espetadores, com uma cada vez maior componente emocional, que deriva da transposição para a indústria do espetáculo, de façanhas extraordinárias, por exemplo com personagens...

Synthesis, characterisation and luminescent properties of lanthanide-organic po...

Three new lanthanide(III) complexes (Ln = Sm, Tb and Eu) of picolinic and glutaric acid were prepared and characterised. The crystal structure of the complex [Sm(glu)(pic)- (H2O)2] (where Hpic and H2glu stand for picolinic and glutaric acid, respectively) was determined by single-crystal Xray diffraction. All the Ln complexes were characterised by elemental analysis, infrared spectroscopy, X-ray powder diffract...

Insights into phage endolysins

(Bacterio)phages are viruses that specifically infect bacteria, and thus are harmless to humans, animals, and plants. They are the most abundant microorganisms on the planet (estimated to be 1031 on Earth) in a ratio of 10 times more than bacteria (1). Consequently, even rare phage-induced events are frequent at the global level. Therefore, they have a staggering ecological impact on the bacterial population an...