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Sexual reproduction of the pentaploid, short-styled Oxalis pes-caprae allows th...

Reproduction is a key factor for the successful establishment and spread of introduced species. Oxalis pes-caprae is a tristylous species with a self- and morph-incompatibility sexual system that, in the invaded range of the western Mediterranean Basin, has been found to reproduce asexually because only the pentaploid, short-styled morph (5x S-morph) was introduced. The objective of this study was to test the a...

Outcomes of Extensive Hybridization and Introgression in Epidendrum (Orchidacea...

Hybridization has the potential to contribute to phenotypic and genetic variation and can be a major evolutionary mechanism. However, when hybridization is extensive it can also lead to the blurring of species boundaries and the emergence of cryptic species (i.e., two or more species not distinguishable morphologically). In this study, we address this hypothesis in Epidendrum, the largest Neotropical genus of o...

Genome size variation and polyploidy incidence in the alpine flora from Spain

The interest to study genome evolution, in particular genome size variation and polyploid incidence, has increased in recent years. Still, only a few studies have been focused at a community level. Of particular interest are high mountain species, because of the high frequency of narrow endemics and their higher susceptibility to extinction due to the effects of climate change. In the present study we explored ...

Effect of invader removal: pollinators stay but some native plants miss their n...

Removal of invasive species often benefits biological diversity allowing ecosystems’ recovery. However, it is important to assess the functional roles that invaders may have established in their new areas to avoid unexpected results from species elimination. Invasive animal-pollinated plants may affect the plant–pollination interactions by changing pollinator availability and/or behaviour in the community. Thus...

So many visitors and so few pollinators: variation in insect frequency and effe...

Plant–pollinator interactions are one of the most important and variable mutualisms having major implications for plant fitness. The present study evaluates the interactions between an endemic milkwort, Polygala vayredae, and its floral visitors by studying the temporal variability, foraging behaviour and effectiveness of floral visitors in three populations during three consecutive years. The flowers were visi...

Flow cytometry as a tool to assess the effects of gamma radiation on the viabil...

Flow cytometry is often used for viability and vitality assessment in bacteria and yeasts. However, its application to the study of fungal spore development is uncommon, probably due to the difficulties in successfully staining these cells. In the current study, we used flow cytometry for the first time to assess the effects of a disinfection treatment on the survival, growth and metabolic activity of fungal sp...

Ploidy stability in embryogenic cultures and regenerated plantlets of tamarillo

Ploidy levels of short-term (1 and 2 years) and long-term (7 and 10 years) embryogenic cultures as well as of regenerated plantlets of tamarillo were analyzed by flow cytometry and chromosome counts. Embryogenic cultures were induced from expanding leaves cultured in the presence of Picloram or 2,4-dichlorophenoxyacetic acid (2,4-D) and monthly subcultured on the same media. Embryo development and plantlets wer...

Apresentação neurológica rara de endocardite infecciosa

Behaviour of human erythrocyte aggregation in presence of autologous lipoproteins

The aim of this work was to evaluate in vitro the effect of autologous plasma lipoprotein subfractions on erythrocyte tendency to aggregate. Aliquots of human blood samples were enriched or not (control) with their own HDL-C, LDL-C, or VLDL-C fractions obtained from the same batch by density gradient ultracentrifugation. Plasma osmolality and erythrocyte aggregation index (EAI) were determined. Blood aliquots e...

Healthy bread as an alternative to traditional bread

Today, bread is part of the culture and eating habits of most people and can be considered one of the most consumed foods in the world. The bread is made from a simple mixture of flour, water, salt and yeast, and it is an extremely versatile food and with a very good digestibility. Since it was first produced for thousands of years, it has undergone changes both ...