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Recent Developments in Molecular Dynamics Simulations of Fluorescent Membrane P...
Loura, Luís M. S.; Ramalho, J. P. PratesDue to their sensitivity and versatility, the use of fluorescence techniques in membrane biophysics is widespread. Because membrane lipids are non-fluorescent, extrinsic membrane probes are widely used. However, the behaviour of these probes when inserted in the bilayer is often poorly understood, and it can be hard to distinguish between legitimate membrane properties and perturbation resulting from probe inco...
Avaliação de propriedades estruturais de membranas lipídicas após substituição ...
Robalo, João R.; Canto, António M. T. Martins do; Carvalho, A. J. Palace; Ramalho, J. P. Prates; Loura, Luís M. S.A espectroscopia e a microscopia de fluorescência têm sido usadas em biofísica de membranas há décadas. Como a unidade estrutural básica das membranas biológicas é a bicamada de lípidos e estes não fluorescem, o uso de sondas extrínsecas de membrana é uma necessidade. Contudo, duas questões preocupantes se levantam quanto ao uso de sondas extrínsecas de fluorescência em estudos de membranas. Em primeiro lugar, ...
Is There a Preferential Interaction between Cholesterol and Tryptophan Residues...
Holt, Andrea; Almeida, Rodrigo F. M. de; Nyholm, Thomas K. M.; Loura, Luís M. S.; Daily, Anna E.; Staffhorst, Rutger W. H. M.; Rijkers, Dirk T. S.Recently, several indications have been found that suggest a preferential interaction between cholesterol and tryptophan residues located near the membrane−water interface. The aim of this study was to investigate by direct methods how tryptophan and cholesterol interact with each other and what the possible consequences are for membrane organization. For this purpose, we used cholesterol-containing model membr...
Effects of fluorescent probe NBD-PC on the structure, dynamics and phase transi...
Loura, Luís M. S.; Fernandes, Fábio; Fernandes, A. C.; Ramalho, J. P. PratesWe present a combined theoretical (molecular dynamics, MD) and experimental (differential scanning calorimetry, DSC) study of the effect of 7-nitrobenz-2-oxa-1,3-diazol-4-yl (NBD) acyl chain-labeled fluorescent phospholipid analogs (C6-NBD-PC and C12-NBD-PC) on 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) bilayers. DSC measurements reveal that <Â 1Â mol% of NBD-PC causes elimination of the pre-transition ...
T-20 and T-1249 HIV fusion inhibitors' structure and conformation in solution: ...
Canto, António M. T. Martins Do; Carvalho, A. J. Palace; Ramalho, J. P. Prates; Loura, Luís M. S.Fusion of the HIV envelope with the target cell membrane is a critical step of the HIV entry into the target cell. Several peptides based on the C-region of HIV gp41 have been used in clinical trials as possible HIV fusion inhibitors. Among these are T-1249 and T-20 (also known as enfurvitide). Despite recent works, a detailed molecular picture of the inhibitory mechanism of these molecules is still lacking. Th...
Sifuvirtide Screens Rigid Membrane Surfaces. Establishment of a Correlation bet...
Franquelim, Henri G.; Loura, Luís M. S.; Santos, Nuno C.; Castanho, Miguel A. R. B.Sifuvirtide, a 36 amino acid negatively charged peptide, is a novel and promising HIV fusion inhibitor, presently in clinical trials. Because of the aromatic amino acid residues of the peptide, its behavior in aqueous solution and the interaction with lipid-membrane model systems (large unilammelar vesicles) were studied by using mainly fluorescence spectroscopy techniques (both steady-state and time-resolved)....
Ciprofloxacin interactions with bacterial protein OmpF: Modelling of FRET from ...
Fernandes, Fábio; Neves, Patrícia; Gameiro, Paula; Loura, Luís M. S.; Prieto, ManuelThe outer membrane protein F (OmpF) is known to play an important role in the uptake of fluoroquinolone antibiotics by bacteria. In this study, the degree of binding of the fluoroquinolone antibiotic ciprofloxacin to OmpF in a lipid membrane environment is quantified using a methodology based on Förster resonance energy transfer (FRET). Analysis of the fluorescence quenching of OmpF is complex as each OmpF mono...
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