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Antimicrobial pressure of ciprofloxacin and gentamicin on biofilm development b...

This work aims at characterizing endoscope biofilm-isolated (PAI) and 2 reference strain P. aeruginosa (PA) adhesion, biofilm formation and sensitivity 3 to antibiotics. The recovery ability of the biofilm-growing bacteria subjected to 4 intermittent antibiotic pressure [ciprofloxacin (CIP) and gentamicin (GM)], as 5 well as the development of resistance towards antibiotics and benzalkonium 6 chloride (BC), wer...

Impact of hypoxic and anaerobic environments on multidrug-resistance of emergin...

OBJECTIVES: It is well-known the establishment of steep oxygen gradients in cystic fibrosis (CF) airways mucus, giving rise to hypoxic or anaerobic zones in the deeper mucus layers, where traditional and atypical bacteria may accommodate and proliferate to biofilms. This study aimed to compare the influence of low-oxygen atmospheres on biofilm growth and susceptibility profiles of the CF-atypical species Inquil...

A colistin coating to prevent biomaterial-associated infections

Synergistic interactions within polymicrobial biofilms formed by atypical and c...

A complex microbial ecosystem is present in Cystic fibrosis (CF)-airways, with uncommon species co-existing and establishing dynamic interactions with traditional pathogens. This study aimed to examine in vitro biofilm formation and susceptibility patterns of two CF-atypical bacteria, Inquilinus limosus (IL) and Dolosigranulum pigrum (DP), when associated to P. aeruginosa (PA), under oxygen-atmospheres resembli...

Antibiotic resistance of mixed biofilms in cystic fibrosis : impact of emerging...

Cystic fibrosis (CF) is a genetic disorder associated with multispecies infections where interactions between classical and newly identified bacteria might be crucial to understanding the persistent colonisation in CF lungs. This study investigated the interactions between two emerging species, Inquilinus limosus and Dolosigranulum pigrum, and the conventional CF pathogen Pseudomonas aeruginosa by evaluating th...

Adaptive response of single and binary Pseudomonas aeruginosa and Escherichia c...

The main goal of this work was to examine whether the continuous exposure of single and binary P. aeruginosa and E. coli biofilms to sub-lethal benzalkonium chloride (BC) doses can induce adaptive response of bacteria. Biofilms were formed during 24 h and then put continuously in contact with BC for more 5 days. The six-day-old adapted biofilms were then submitted to BC challenge, characterized and inspected by...

Role of oxygen-restricted environments in biofilm growth and susceptibility pro...

It has been widely demonstrated that different predisposing “local environmental” conditions exist within the cystic fibrosis (CF) airways. Steep oxygen gradients are frequently detected in CF airways and may give rise to hypoxic or even anaerobic zones, providing nutritionally rich growth environments where bacteria may penetrate and accommodate, and being also responsible for the increase of antimicrobial res...

Impact of environments with distinct oxygen availability on biofilm growth and ...

One of the main manifestations of cystic fibrosis (CF) is a decreased clearance of mucus with concomitant bacterial lung infections. Infections are caused by a wide variety of organisms, which includes not only the typical “residents” but also other uncommon bacteria. It has been widely demonstrated that steep oxygen gradients exist within the mucus layers of cystic fibrosis, giving rise to hypoxic/anaerobic ar...

Characterization of the virulence and the antibiotic susceptibility profiles in...

The increased prevalence of antibiotic resistance is one of the main problems faced by health professionals nowadays, consequently dealing with the expansion of nosocomial infections in hospitals and other health institutions. Wastewater Treatment Plants (WWTP) aim at the treatment of residual waters ensuring the preservation of the environment and ultimately, human health. Nevertheless, they also receive resid...

Effective association of tea tree essential oil with conventional antibiotics t...

Pseudomonas aeruginosa is an important cause of acute nosocomial and biofilm-associated infections that are often difficult to eradicate. The increase of antimicrobial resistance, especially in sessile microorganisms, has promoted the search for new alternatives, as natural products, to control biofilm infections. Essential oils are natural, volatile, and complex compounds produced by aromatic plants as seconda...