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Characterization of non-conventional yeasts under hyperbaric conditions : cellu...

Tese de doutoramento em Chemical and Biological Engineering ; Providing an adequate oxygen supply is critical to the growth and maintenance of most aerobic microbial cultures used in biotechnological processes. Oxygen mass transfer from gas phase to the culture medium is often a major growth limiting factor because of oxygen´s low solubility in an aqueous solution. Thus, ensuring adequate oxygen supply to subm...

Comparison of Yarrowia lipolytica and Pichia pastoris cellular response to diff...

Yeast cells exposed to adverse conditions employ a number of defense mechanisms in order to respond effectively to the stress effects of reactive oxygen species. In this work, the cellular response of Yarrowia lipolytica and Pichia pastoris to the exposure to the ROSinducing agents’ paraquat, hydrogen peroxide, and increased air pressure was analyzed. Yeast cells at exponential phase were exposed for 3 h to 1 m...

Batch and fed-batch growth of Pichia pastoris under increased air pressure

Pichia pastoris CBS 2612 behavior under air pressures of 1 bar, 3 bar and 5 bar in culture media of glycerol (pure and crude) and methanol was studied. Generally, the increase in oxygen transfer rate due to the increase of total pressure improved cellular growth for all carbon sources and for batch and fed-batch processes with different feeding rate strategies. In batch cultures, 1.4-fold, 1.2-fold, and 1.5-fol...

Erratum to : Batch and fed-batch growth of Pichia pastoris under increased air ...

Oxygen mass transfer rate in a pressurized lab-scale stirred bioreactor

Oxygen mass transfer from air to the liquid phase in bioreactors with aerobic cultures has long been a serious impairment to the productivity of various bioprocesses. An increase of the oxygen mass transfer rate (OTR) can be the key to overcome oxygen limitation. The influence of higher air pressure on OTR was measured and a significantly enhanced OTR could be obtained. The oxygen volumetric mass transfer coeff...

Expression of heterologous proteins by Pichia pastoris under increased air pres...

The P. pastoris expression system has gained acceptance as an important host organism for the production of heterologous proteins. Its expression system presents the advantage of the organism can grow to the desired cell density on glycerol as the carbon source and then on methanol for high level heterologous production. However, the oxygen limitation generally has a detrimental effect on the expression of for...

Prática de ensino supervisionada em Educação Pré-Escolar e Ensino do 1º Ciclo d...

Neste relatório pretende-se descrever e refletir sobre a ação educativa desenvolvida no âmbito da Prática de Ensino Supervisionada, em contexto pré-escolar e de 1.ºCiclo do Ensino Básico, entre fevereiro e junho de 2012 e, outubro e janeiro de 2013, respetivamente. Apresenta-se a contextualização das instituições localizadas numa zona urbana, a respetiva caraterização dos grupos de crianças, que integram idades...

Estágio pedagógico na UTAD “ Em Bolonha”: revisão bibliográfica

O objetivo deste trabalho foi a verificação da Didática como aspeto mais relevante e responsável pelo efeito da aprendizagem, onde o feedback pedagógico é um dos fatores que desempenham um dos papéis mais importantes na prática pedagógica. Ao longo do trabalho é dado relevo aos conceitos de didática enquanto ferramenta e ciência cujo objeto de estudo se centra nas estratégias de ensino-aprendizagem, supervisão ...

Application of hyperbaric bioreactors for bioprocess development

For many years pressure was disregarded by biotechnologists when considering processes based on microbial cultures, due to the idea of incompatibility of life in pressure environments above atmospheric pressure. However, the discovery of microbial life in such environments has refuted this idea. Moreover, in industrial bioreactors microorganisms are exposed to spatial gradients of pressure (with average values ...

Images of aging in institutionalized and non-institutionalized elderly people

Aim: The aim of this study was to assess and compare images and stereotypes of aging in institutionalized and non-institutionalized elderly people. This is a descriptive study using a survey. Method: The instruments used were a sociodemographic questionnaire and the ImAges scale. A total of 120 elderly people, aged between 65 and 99 years, participated in this study. Results: Statistically significant differenc...