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Analysis of allelic imbalances and protein expression in human high-grade astro...

DNFB activates MAPKs and upregulates CD40 in skin-derived dendritic cells

The intracellular mechanisms involved in the activation of DCs during sensitization in allergic contact dermatitis (ACD) are not known.Objective: ; http://www.sciencedirect.com/science/article/B6T87-4G65C97-1/1/ed9bb97edaedb94bbcf0ea48b20f28a3

Contact sensitizer nickel sulfate activates the transcription factors NF-kB and...

Nuclear factor kappa B (NF-kB) and activating protein-1 (AP-1) transcription factors are ubiquitously expressed signaling molecules known to regulate the transcription of a large number of genes involved in immune responses, namely the inducible isoform of nitric oxide synthase (iNOS). In this study, we demonstrate that a fetal skin-derived dendritic cell line (FSDC) produces nitric oxide (NO) in response to th...

Differential roles of hydrogen peroxide and superoxide in mediating IL-1-induce...

Our previous studies showed that reactive oxygen species (ROS) are required for the pro-inflammatory cytokine interleukin-1beta (IL-1) to induce the activity of the Nuclear transcription Factor-kappaB (NF-kappaB) and the expression of the inducible isoform of the nitric oxide synthase (iNOS) in bovine articular chondrocytes. This study aimed at elucidating the role of hydrogen peroxide (H2O2) and the superoxide...

Dexamethasone prevents granulocyte-macrophage colonystimulating factor-induced ...

AIMS: Nitric oxide (NO) has been increasingly implicated in inflammatory skin diseases, namely in allergic contact dermatitis. In this work, we investigated the effect of dexamethasone on NO production induced by the epidermal cytokine granulocyte / macrophage colony-stimulating factor (GM-CSF) in a mouse fetal skin dendritic cell line.Methods: NO production was assessed by the method of Griess. Expression of t...

Granulocyte-macrophage colony-stimulating factor activates the transcription of...

Nitric oxide (NO) produced by skin dendritic cells and keratinocytes plays an important role in skin physiology, growth and remodelling. Nitric oxide is also involved in skin inflammatory processes and in modulating antigen presentation (either enhancing or suppressing it). In this study, we found that GM-CSF stimulates the expression of the inducible isoform of nitric oxide synthase (iNOS) in a fetal-skin-deri...

17beta-estradiol promotes the synthesis and the secretion of annexin I in the C...

AIMS: Annexin I (ANXA1), a 37kDa member of the annexin family of Ca2+-binding and phospholipid-binding proteins, is particularly abundant in various populations of peripheral blood leukocytes. Since this protein modulates the anti-inflammatory actions of the steroid hormones, the purpose of this study was to investigate the effects of the female sex steroid hormone, 17beta-estradiol (E2beta), on the synthesis a...

Diphenyleneiodonium inhibits NF-kappaB activation and iNOS expression induced b...

AIMS: In this work, we studied the mechanisms by which diphenyleneiodonium chloride (DPI) inhibits nitric oxide (NO) synthesis induced by the proinflammatory cytokine interleukin-1beta (IL-1) in bovine articular chondrocytes. To achieve this, we evaluated the ability of DPI to inhibit the expression and activity of the inducible isoform of the NO synthase (iNOS) induced by IL-1. We also studied the ability of D...

Ca2(+)-dependent binding of tamoxifen to calmodulin isolated from bovine brain

The interaction of the antiestrogen tamoxifen (Tx) with calmodulin (CaM) was investigated by cross-linking between the protein and [3H] tamoxifen aziridine. We observed that CaM binds Tx in a Ca2(+)-dependent manner and that two components are involved in the binding, with apparent dissociation constants (Kd) of about 6 nM and 9 microM. The high affinity binding site has a maximal capacity of 25 pmol/mg protein...