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The perpetual movements of anaphase
Maiato, Helder; Lince-Faria, MarianaOne of the most extraordinary events in the lifetime of a cell is the coordinated separation of sister chromatids during cell division. This is truly the essence of the entire mitotic process and the reason for the most profound morphological changes in cytoskeleton and nuclear organization that a cell may ever experience. It all occurs within a very short time window known as “anaphase”, as if the cell had spe...
Synchronizing chromosome segregation by flux-dependent force equalization at ki...
Matos, Irina; Pereira, António J.; Lince-Faria, Mariana; Cameron, Lisa A.; Salmon, Edward D.; Maiato, HelderThe synchronous movement of chromosomes during anaphase ensures their correct inheritance in every cell division. This reflects the uniformity of spindle forces acting on chromosomes and their simultaneous entry into anaphase. Although anaphase onset is controlled by the spindle assembly checkpoint, it remains unknown how spindle forces are uniformly distributed among different chromosomes. In this paper, we sh...
Spatiotemporal control of mitosis by the conserved spindle matrix protein Megator
Lince-Faria, Mariana; Maffini, Stefano; Orr, Bernard; Ding, Yun; Florindo, Cláudia; Sunkel, Claudio E.; Tavares, Álvaro; Johansen, JørgenA putative spindle matrix has been hypothesized to mediate chromosome motion, but its existence and functionality remain controversial. In this report, we show that Megator (Mtor), the Drosophila melanogaster counterpart of the human nuclear pore complex protein translocated promoter region (Tpr), and the spindle assembly checkpoint (SAC) protein Mad2 form a conserved complex that localizes to a nuclear derived...
Chromator is required for proper microtubule spindle formation and mitosis in D...
Ding, Yun; Yao, Changfu; Lince-Faria, Mariana; Rath, Uttama; Cai, Weili; Maiato, Helder; Girton, Jack; Johansen, Kristen M.; Johansen, JørgenThe chromodomain protein, Chromator, has been shown to have multiple functions that include regulation of chromatin structure as well as coordination of muscle remodeling during metamorphosis depending on the developmental context. In this study we show that mitotic neuroblasts from brain squash preparations from larvae heteroallelic for the two Chromator lossof- function alleles Chro71 and Chro612 have severe ...
Dissecting mitosis with laser microsurgery and RNAi in Drosophila cells
Pereira, António J.; Matos, Irina; Lince-Faria, Mariana; Maiato, HelderProgress from our present understanding of the mechanisms behind mitosis has been compromised by the fact that model systems that were ideal for molecular and genetic studies (such as yeasts, C. elegans or Drosophila) were not suitable for intracellular micromanipulation. Unfortunately, those systems that were appropriate for micromanipulation (like newt lung cells, PtK1 cells or insect spermatocytes) are not a...
Mammalian CLASP1 and CLASP2 cooperate to ensure mitotic fidelity by regulating ...
Pereira, Ana L.; Pereira, António J.; Maia, Ana R.R.; Drabek, Ksenija; Sayas, C. Laura; Hergert, Polla J.; Lince-Faria, Mariana; Matos, IrinaCLASPs are widely conserved microtubule plus-end–tracking proteins with essential roles in the local regulation of microtubule dynamics. In yeast, Drosophila, and Xenopus, a single CLASP orthologue is present, which is required for mitotic spindle assembly by regulating microtubule dynamics at the kinetochore. In mammals, however, only CLASP1 has been directly implicated in cell division, despite the existence ...
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