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Self mutagens affect detrimentally PCR analysis of food fungi by creating poten...
Paterson, R. R. M.; Lima, NelsonVersão aceite em 2013 ; Analysing fungi from food by PCR is increasing rapidly. However, food fungi produce mutagens which may mutate the fungi in culture so that fungi which produce mycotoxins may have a negative PCR result for genes in the mycotoxin metabolic pathway and vice versa: It is impossible to state unequivocally that the current PCR results obtained are accurate. For example, food containing a myco...
The use of MALDI-TOF ICMS as an alternative tool for Trichophyton rubrum identi...
Pereira, Leonel; Dias, Nicole; Santos, Cledir; Lima, NelsonIn this study, the potential of matrix-assisted laser desorption/ionization time-of-flight intact cell mass spectrometry (MALDI-TOF ICMS) was investigated for the identification of clinical isolates. The isolates were analyzed at the species and strain level,
An overview of the recent developments on fructooligosaccharide production and ...
Dominguez, Ana; Rodrigues, L. R.; Lima, Nelson; Teixeira, J. A.Over the past years, many researchers have suggested that deficiencies in the diet can lead to disease states and that some diseases can be avoided through an adequate intake of relevant dietary components. Recently, a great interest in dietary modulation of the human gut has been registered. Prebiotics, such as fructooligosaccharides (FOS), play a key role in the improvement of gut microbiota balance and in in...
Degradation of Metalaxyl and Folpet by Filamentous Fungi Isolated From Portugue...
Martins, M. Rosário; Pereira, Pablo; Lima, Nelson; Cruz-Morais, JúlioDegradation of xenobiotics by microbial populations is a potential method to enhance the effectiveness of ex situ or in situ bioremediation. The propose of this study was to evaluate the impact of repeated metalaxyl and folpet treatments on soil microbial communities and to select soil fungal strains able to degrade these fungicides. Results showed an enhanced degradation of metalaxyl and folpet in vineyards so...
White-rot fungi capable of decolourising textile dyes under alkaline conditions
Ottoni, C. A.; Santos, C.; Kozakiewicz, Z.; Lima, NelsonTwelve white-rot fungal strains belonging to seven different species were screened on plates under alkaline condition to study the decolourisation of the textile dyes Reactive Black 5 and Poly R-478. Three strains of Trametes versicolor (Micoteca da Universidade do Minho (MUM) 94.04, 04.100 and 04.101) and one strain of Phanerochaete chrysosporium (MUM 94.15) showed better decolourisation results. These four st...
Biochemical mutagens affect the preservation of fungi and biodiversity estimations
Paterson, R. R. M.; Lima, NelsonMany fungi have significant industrial applications or biosafety concerns and maintaining the original characteristics is essential. The preserved fungi have to represent the situation in nature for posterity, biodiversity estimations, and taxonomic research. However, spontaneous fungal mutations and secondary metabolites affecting producing fungi are well known. There is increasing interest in the preservation...
Interaction with Penicillium expansum enhances Botrytis cinerea growth in grape...
Morales-Valle, H.; Paterson, R. R. M.; Venâncio, Armando; Lima, NelsonInteractions between fungi occur when they grow on the same host plant. This is the case of Botrytis cinerea and Penicillium expansum on grape. P. expansum is also responsible for production of the mycotoxin patulin. In this study, the influence of the interaction between both fungi on fungal growth parameters was studied as well as the effect on the accumulation of patulin by P. expansum. For that purpose, spo...
How will climate change affect oil palm fungal diseases?
Paterson, R. R. M.; Sariah, M.; Lima, NelsonPalm oil is a very important commodity. It is added to numerous products and is a biofuel. However, oil palms (OP) are subjected to fungal diseases of which Fusarium wilt and Ganoderma rots are the most important. Considerations of how climate change (CC) affects tropical economic plants are limited and for OP are even fewer. The margin for adapting to higher temperatures and changing humidity is reduced in tro...
Biofilms from a Brazilian water distribution system include filamentous fungi
Siqueira, Virgínia M.; Oliveira, H. M. B.; Santos, C.; Paterson, R. R. M.; Gusmão, N. B.; Lima, NelsonFilamentous fungi in drinking water can block water pipes, can cause organoleptic biodeterioration, and are a source of pathogens. There are increasing reports of the involvement of the organisms in biofilms. This present study describes a sampling device that can be inserted directly into pipes within water distribution systems, allowing biofilm formation in situ. Calcofluor White M2R staining and fluorescent ...
Projeto e desenvolvimento de um equipamento triturador de vidro
Lima, Nélson Natanael FerreiraDissertação de mestrado integrado em Engenharia Mecânica ; Hoje em dia, em Portugal mas em mais relevância em países como Inglaterra, a reciclagem é um serviço indispensável no funcionamento do país. Daí que surge um problema quanto ao transporte das embalagens de vidro, pois estas por norma têm um volume diversas vezes superior ao que volume de material que têm. Os vidrões estão equipados com um sistema que p...
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