Encontrados 18 documentos, a visualizar página 1 de 2
S4(13)-PV cell-penetrating peptide induces physical and morphological changes i...
Cardoso, Ana M. S.; Trabulo, Sara; Cardoso, Ana L.; Lorents, Annely; Morais, Catarina M.; Gomes, Paula; Nunes, Cláudia; Lúcio, Marlene; Reis, SaletteThe present work aims to gain insights into the role of peptide–lipid interactions in the mechanisms of cellular internalization and endosomal escape of the S4(13)-PV cell-penetrating peptide, which has been successfully used in our laboratory as a nucleic acid delivery system. A S4(13)-PV analogue, S4(13)-PVscr, displaying a scrambled amino acid sequence, deficient cell internalization and drug delivery inabil...
Towards a siRNA-containing nanoparticle targeted to breast cancer cells and the...
Gomes-da-Silva, Lígia C.; Santos, Adriana O.; Bimbo, Luís M.; Moura, Vera; Ramalho, José S.; Lima, Maria C. Pedroso de; Simões, Sérgio; Moreira, João N.The present work aimed at designing a lipid-based nanocarrier for siRNA delivery towards two cell sub-populations within breast tumors, the cancer and the endothelial cells from angiogenic tumor blood vessels. To achieve such goal, the F3 peptide, which is specifically internalized by nucleolin overexpressed on both those sub-populations, was used as a targeting moiety. The developed F3-targeted stable nuc...
Suicide gene therapy in cancer: Where do we stand now?
Duarte, Sónia; Carle, Georges; Faneca, Henrique; Lima, Maria C. Pedroso de; Pierrefite-Carle, ValérieSuicide gene therapy is based on the introduction into tumor cells of a viral or a bacterial gene, which allows the conversion of a non-toxic compound into a lethal drug. Although suicide gene therapy has been successfully used in a large number of in vitro and in vivo studies, its application to cancer patients has not reached the desirable clinical significance. However, recent reports on pre-clinical cancer ...
Cell-Penetrating Peptides - Mechanisms of Cellular Uptake and Generation of Del...
Trabulo, Sara; Cardoso, Ana Luísa; Mano, Miguel; Lima, Maria C. Pedroso deThe successful clinical application of nucleic acid-based therapeutic strategies has been limited by the poor delivery efficiency achieved by existing vectors. The development of alternative delivery systems for improved biological activity is, therefore, mandatory. Since the seminal observations two decades ago that the Tat protein, and derived peptides, can translocate across biological membranes, cell-penetr...
African swine fever virus p10 protein exhibits nuclear import capacity and accu...
Nunes-Correia, Isabel; Rodríguez, Javier M.; Eulálio, Ana; Carvalho, Ana L.; Citovsky, Vitaly; Simões, Sérgio; Faro, Carlos; Salas, María L.African swine fever virus (ASFV), a large enveloped DNA-containing virus, infects domestic and wild pigs, and multiplies in soft ticks, causing an economically relevant hemorrhagic disease. Evaluation of the nuclear import ability of ASFV p10 protein was the major purpose of the present work. Two approaches were used to determine if p10 protein is imported into the nucleus by an active process: a yeast-based nu...
S4 13 - PV cell penetrating peptide and cationic liposomes act synergistically...
Trabulo, Sara; Mano, Miguel; Faneca, Henrique; Cardoso, Ana Luísa; Duarte, Sónia; Henriques, Ana; Paiva, Artur; Gomes, Paula; Simões, SérgioCell penetrating peptides have been successfully used to mediate the intracellular delivery of a wide variety of molecules of pharmacological interest. The main aim of the present work was to evaluate the potential of the S413-PV cell penetrating peptide to mediate the intracellular delivery of plasmid DNA, aiming at its use in gene therapy applications. The S413-PV cell penetrating peptide is a chimeric peptid...
Striatal and nigral pathology in a lentiviral rat model of Machado-Joseph disease
Alves, Sandro; Régulier, Etienne; Nascimento-Ferreira, Isabel; Hassig, Raymonde; Dufour, Noelle; Koeppen, Arnulf; Carvalho, Ana Luísa; Simões, SérgioMachado-Joseph disease (MJD) is a fatal, dominant neurodegenerative disorder. MJD results from polyglutamine repeat expansion in the MJD-1 gene, conferring a toxic gain of function to the ataxin-3 protein. In this study, we aimed at overexpressing ataxin-3 in the rat brain using lentiviral vectors (LV), to generate an in vivo MJD genetic model and, to study the disorder in defined brain regions: substantia nigr...
Allele-specific RNA silencing of mutant ataxin-3 mediates neuroprotection in a ...
Alves, Sandro; Nascimento-Ferreira, Isabel; Auregan, Gwennaëlle; Hassig, Raymonde; Dufour, Noëlle; Brouillet, Emmanuel; Lima, Maria C. Pedroso deRecent studies have demonstrated that RNAi is a promising approach for treating autosomal dominant disorders. However, discrimination between wild-type and mutant transcripts is essential, to preserve wild-type expression and function. A single nucleotide polymorphism (SNP) is present in more than 70% of patients with Machado-Joseph disease (MJD). We investigated whether this SNP could be used to inactivate mut...
Transferrin-Associated Lipoplexes as Gene Delivery Systems: Relevance of Mode o...
Penacho, Nuno; Filipe, Ana; Simões, Sérgio; Lima, Maria C. Pedroso deAbstract The successful application of gene therapy depends highly on understanding the properties of gene carriers and their correlation with the ability to mediate transfection. An important parameter that has been described to improve transfection mediated by cationic liposomes involves association of ligands to cationic liposome–DNA complexes (lipoplexes). In this study, ternary complexes composed of 1,2-...
Interaction of S413-PV cell penetrating peptide with model membranes: relevance...
Mano, Miguel; Henriques, Ana; Paiva, Artur; Prieto, Manuel; Gavilanes, Francisco; Simões, Sérgio; Lima, Maria C. Pedroso DeCell penetrating peptides (CPPs) have been successfully used to mediate the intracellular delivery of a wide variety of molecules of pharmacological interest both in vitro and in vivo, although the mechanisms by which the cellular uptake occurs remain unclear and controversial. Following our previous work demonstrating that the cellular uptake of the S413-PV CPP occurs mainly through an endocytosis-independent ...
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