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Synergistic antitumoral effect of vinblastine and HSV-Tk/GCV gene therapy media...
Faneca, H.; Faustino, A.; Lima, M. C. Pedroso deDespite recent advances in conventional therapeutic approaches for cancer, the frequently observed acquired drug resistance and toxic side effects have limited their clinical application. The main goal of this work was to investigate the combined antitumoral effect of vinblastine with HSV-Tk/GCV "suicide" gene therapy mediated by human serum albumin (HSA)-associated lipoplexes, in mammary adenocarcinoma cells (...
Simultaneous evaluation of viability and Bcl-2 in small-cell lung cancer
Santos, A.; Ribeiro, A. B. Sarmento; Lima, M. C. Pedroso de; Simões, S.; Moreira, J. N.When designing molecular targeted therapeutic strategies against cancer, it is important to correlate protein expression and cell viability. However, such goal can be difficult if performed in separate assays, especially when only a fraction of cells has been efficiently transfected. Therefore, the aim of the present study was to establish a flow cytometry procedure to assess simultaneously Bcl-2 protein level ...
Tf-lipoplexes for neuronal siRNA delivery: A promising system to mediate gene s...
Cardoso, A. L. C.; Simões, S.; Almeida, L. P. de; Plesnila, N.; Lima, M. C. Pedroso de; Wagner, E.; Culmsee, C.http://www.sciencedirect.com/science/article/B6T3D-4TBXG8W-1/2/0126aa3460b30ae6bbcf04124c1c63dd
Evaluation of the antitumoral effect mediated by IL-12 and HSV-tk genes when de...
Faneca, H.; Cabrita, A. S.; Simões, S.; Lima, M. C. Pedroso deIn the present work, we used a novel albumin-associated lipoplex formulation, containing the cationic lipid 1-palmitoyl-2-oleoyl-sn-glycero-3-ethylphosphocholine (EPOPC) and cholesterol (Chol), to evaluate the antitumoral efficacy of two gene therapy strategies: immuno-gene therapy, mediated by IL-12 gene expression, and "suicide" gene therapy, mediated by HSV-tk gene expression followed by ganciclovir (GCV) tr...
siRNA delivery by a transferrin-associated lipid-based vector: a non-viral stra...
Cardoso, A. L. C.; Simões, S.; Almeida, L. P. de; Pelisek, J.; Culmsee, C.; Wagner, E.; Lima, M. C. Pedroso deRNA interference provides a powerful technology for specific gene silencing. Therapeutic applications of small interfering RNA (siRNA) however require efficient vehicles for stable complexation, protection, and extra- and intracellular delivery of these nucleic acids. Here, we evaluated the potential of transferrin (Tf)-associated liposomes for siRNA complexation and gene silencing.Cationic liposomes composed o...
Electrochemical sensing of the behaviour of oligonucleotide lipoplexes at charg...
Piedade, J. A. P.; Mano, M.; Lima, M. C. Pedroso de; Oretskaya, T. S.; Oliveira-Brett, A. M.Complexes between short oligodeoxynucleotides (ODN) with a variable dGxdCy base composition and liposomes composed of the cationic lipid DOTAP (ODN lipoplexes) were studied by differential pulse voltammetry at a glassy carbon electrode. Since lipoplexes are spontaneously formed by electrostatic interactions, the objective of the voltammetric study was to investigate their behaviour at the electrode surface/solu...
Voltammetric behaviour of oligonucleotide lipoplexes adsorbed onto glassy carbo...
Piedade, J. A. P.; Mano, M.; Lima, M. C. Pedroso de; Oretskaya, T. S.; Oliveira-Brett, A. M.The voltammetric behaviour of oligonucleotide lipoplexes (ODN-lipoplexes) prepared from short oligodeoxynucleotides (ODN), with different base compositions, and liposomes of the cationic lipid DOTAP, was studied by differential pulse voltammetry with a glassy carbon mini-electrode. It was found that the ODN base composition influences the ODN-lipoplex voltammetric response. Differential pulse voltammograms for ...
Association of albumin or protamine to lipoplexes: enhancement of transfection ...
Faneca, H.; Simões, S.; Lima, M. C. Pedroso deThe successful application of gene therapy depends on the availability of carriers to efficiently deliver genetic material into target cells. Such efficacy is strongly related to key parameters including serum resistance and protection of DNA.The complexes were tested in terms of their biological activity, in the absence or presence of serum, by following transfection activity. Interaction with plasma proteins ...
Two African swine fever virus proteins derived from a common precursor exhibit ...
Eulálio, A.; Nunes-Correia, I.; Carvalho, A. L.; Faro, C.; Citovsky, V.; Simões, S.; Lima, M. C. Pedroso deAfrican swine fever virus (ASFV), a large icosahedral deoxyvirus, is the causative agent of an economically relevant hemorrhagic disease that affects domestic pigs. The major purpose of the present study was to investigate the nuclear transport activities of the ASFV p37 and p14 proteins, which result from the proteolytic processing of a common precursor. Experiments were performed by using yeast-based nucleocy...
Evaluation of lipid-based reagents to mediate intracellular gene delivery
Faneca, H.; Simões, S.; Lima, M. C. Pedroso deWe characterized different cationic lipid-based gene delivery systems consisting of both liposomes and nonliposomal structures, in terms of their in vitro transfection activity, resistance to the presence of serum, protective effect against nuclease degradation and stability under different storage conditions. The effect of lipid/DNA charge ratio of the resulting complexes on these properties was also evaluated...
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